Construct: ORF TRCN0000470061
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013955.1_s317c1
- Derived from:
- ccsbBroadEn_10858
- DNA Barcode:
- TCACAGTTCTTATGGAGGGCTTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPX1 (2876)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470061
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2876 | GPX1 | glutathione peroxidase 1 | NM_001329455.1 | 86.8% | 69.3% | (many diffs) |
| 2 | human | 2876 | GPX1 | glutathione peroxidase 1 | NM_000581.4 | 71.4% | 1% | 1_174del |
| 3 | human | 2876 | GPX1 | glutathione peroxidase 1 | NM_001329503.2 | 50.4% | 1% | 1_174del;251_269del;510_511ins118 |
| 4 | mouse | 14775 | Gpx1 | glutathione peroxidase 1 | NM_001329528.1 | 82.2% | 85.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 501
- ORF length:
- 435
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa cgagctgcag cggcgcctcg gaccccgggg cctggtggtg ctcggcttcc 121 cgtgcaacca gtttGGGCAT CAGGAGAACG CCAAGAACGA AGAGATTCTG AATTCCCTCA 181 AGTACGTCCG GCCTGGTGGT GGGTTCGAGC CCAACTTCAT GCTCTTCGAG AAGTGCGAGG 241 TGAACGGTGC GGGGGCGCAC CCTCTCTTCG CCTTCCTGCG GGAGGCCCTG CCAGCTCCCA 301 GCGACGACGC CACCGCGCTT ATGACCGACC CCAAGCTCAT CACCTGGTCT CCGGTGTGTC 361 GCAACGATGT TGCCTGGAAC TTTGAGAAGT TCCTGGTGGG CCCTGACGGT GTGCCCCTAC 421 GCAGGTACAG CCGCCGCTTC CAGACCATTG ACATCGAGCC TGACATCGAA GCCCTGCTGT 481 CTCAAGGGCC CAGCTGTGCC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 541 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTA 601 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATCAC AGTTCTTATG 661 GAGGGCTTAC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt