Construct: ORF TRCN0000470062
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009117.1_s317c1
- Derived from:
- ccsbBroadEn_07915
- DNA Barcode:
- AGCCAACTATCCGTGATTGGGCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MKRN2 (23609)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470062
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23609 | MKRN2 | makorin ring finger protein 2 | NM_014160.5 | 99.8% | 99.7% | 636T>C;1244A>T |
| 2 | human | 23609 | MKRN2 | makorin ring finger protein 2 | NM_001271707.1 | 89.5% | 89.4% | 21_22ins129;507T>C;1115A>T |
| 3 | mouse | 67027 | Mkrn2 | makorin, ring finger protei... | NM_023290.2 | 84.8% | 88.2% | (many diffs) |
| 4 | mouse | 67027 | Mkrn2 | makorin, ring finger protei... | XM_011241431.1 | 45.3% | 45.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1314
- ORF length:
- 1248
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag caccaagcag atcacttgca ggtattttat gcatggtgtg tgtcgggaag 121 gaagtcagtg cctattctca catgacttgg caaacagcaa accgtccacc atctgcaagt 181 actaccagaa gggctactgt gcctatggaa ctcggtgcag atatgaccac acgaggccct 241 ctgctgcagc tggaggtgct gtgggcacca tggcccacag tgtgccctcc ccagctttcc 301 acagtcctca ccctccttcc gaggtcactg catccattgt gaaaactaac tcacatgaac 361 ccggaaagcg tgaaaagaga acattggttc ttagagaccg aaatctctct ggcatggctg 421 aaaggaagac ccagccgagc atggtgagta atccaggcag ctgcagcgac ccccagccca 481 gccccgagat gaagccgcat tcctacctgg atgccatcag gagtggcctt gatgacgtgg 541 aggccagcag ctcctacagc aacgagcagc agctgtgccc ctacgcagct gctggggagt 601 gccggtttgg ggatgcctgt gtctacctgc acggggaggt gtgtgaaatc tgtaggctgc 661 aagtcttgca cccattcgac ccagagcaga ggaaggctca cgaaaagatc tgcatgttga 721 cgttcgaaca cgagatggaa aaggcctttg ccttccaggc aagccaggac aaagtgtgca 781 gtatctgcat ggaagtgatc ctggagaagg cctctgcttc tgagaggaga tttgggattc 841 tctccaattg caatcacacg tactgtttgt cctgcatccg gcagtggcgg tgtgccaaac 901 agtttGAAAA CCCAATCATT AAGTCTTGTC CAGAATGCCG TGTGATATCA GAGTTTGTAA 961 TTCCAAGTGT GTATTGGGTG GAAGATCAGA ATAAAAAGAA CGAGTTGATT GAAGCTTTCA 1021 AACAGGGGAT GGGGAAAAAA GCCTGTAAAT ACTTTGAGCA AGGCAAGGGG ACCTGCCCAT 1081 TTGGAAGCAA ATGTCTTTAT CGCCATGCTT ACCCCGATGG GCGGCTAGCA GAGCCTGAGA 1141 AACCTCGGAA ACAGCTCAGT TCTCAAGGCA CTGTGAGGTT CTTTAATTCA GTGCGGCTCT 1201 GGGATTTCAT CGAGAACCGA GAAAGCCGGC ATGTCCCCAA CAATGAAGAT GTCGACATGA 1261 CAGAGCTCGG GGACCTCTTC ATGCACCTTT CTGGAGTGGA ATCATCAGTA CCCTACCCAA 1321 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1381 ATTCTACGTA GTAATGAACT AGTCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT 1441 ATCTTGTGGA AAGGACGAAG CCAACTATCC GTGATTGGGC ACACGCGTTA AGTCgacaat 1501 caacctctgg attacaaaat ttgtgaaaga tt