Construct: ORF TRCN0000470075
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006224.1_s317c1
- Derived from:
- ccsbBroadEn_01320
- DNA Barcode:
- CCTTTATGTCATGGCAGCATGCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PSMD4 (5710)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470075
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5710 | PSMD4 | proteasome 26S subunit, non... | NM_002810.3 | 100% | 100% | |
| 2 | human | 5710 | PSMD4 | proteasome 26S subunit, non... | NM_001330692.2 | 99.2% | 99.2% | 762_770delAGGTGAAAG |
| 3 | human | 266971 | PIPSL | PIP5K1A and PSMD4 like (pse... | NR_002319.2 | 28.9% | (many diffs) | |
| 4 | mouse | 19185 | Psmd4 | proteasome (prosome, macrop... | NM_008951.2 | 90.1% | 96% | (many diffs) |
| 5 | mouse | 19185 | Psmd4 | proteasome (prosome, macrop... | NM_001282017.1 | 89.4% | 95.2% | (many diffs) |
| 6 | mouse | 19185 | Psmd4 | proteasome (prosome, macrop... | XM_006501137.2 | 63.8% | 67.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1197
- ORF length:
- 1131
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gttggaaagc actatggtgt gtgtggacaa cagtgagtat atgcggaatg 121 gagacttctt acccaccagg ctgcaggccc agcaggatgc tgtcaacata gtttgtcatt 181 caaagacccg cagcaaccct gagaacaacg tgggccttat cacactggct aatgactgtg 241 aagtgctgac cacactcacc ccagacactg gccgtatcct gtccaagcta catactgtcc 301 aacccaaggg caagatcacc ttctgcacgg gcatccgcgt ggcccatctg gctctgaagc 361 accgacaagg caagaatcac aagatgcgca tcattgcctt tgtgggaagc ccagtggagg 421 acaatgagaa ggatctggtg aaactggcta aacgcctcaa gaaggagaaa gtaaatgttg 481 acattatcaa ttttggggaa gaggaggtga acacagaaaa gctgacagcc tttgtaaaca 541 cgttgaatgg caaagatgga accggttctc atctggtgac agtgcctcct gggcccagtt 601 tggctgatgc tctcatcagt tctccgattt tggctggtga aggtggtgcc atgctgggtc 661 ttggtgccag tgactttgaa tttggagtag atcccagtgc tgatcctgag ctggccttgg 721 cccTTCGTGT ATCTATGGAA GAGCAGCGGC AGCGGCAGGA GGAGGAGGCC CGGCGGGCAG 781 CTGCAGCTTC TGCTGCTGAG GCCGGGATTG CTACGACTGG GACTGAAGAC TCAGACGATG 841 CCCTGCTGAA GATGACCATC AGCCAGCAAG AGTTTGGCCG CACTGGGCTT CCTGACCTAA 901 GCAGTATGAC TGAGGAAGAG CAGATTGCTT ATGCCATGCA GATGTCCCTG CAGGGAGCAG 961 AGTTTGGCCA GGCGGAATCA GCAGACATTG ATGCCAGCTC AGCTATGGAC ACATCTGAGC 1021 CAGCCAAGGA GGAGGATGAT TACGACGTGA TGCAGGACCC CGAGTTCCTT CAGAGTGTCC 1081 TAGAGAACCT CCCAGGTGTG GATCCCAACA ATGAAGCCAT TCGAAATGCT ATGGGCTCCC 1141 TGGCCTCCCA GGCCACCAAG GACGGCAAGA AGGACAAGAA GGAGGAAGAC AAGAAGTGCC 1201 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1261 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1321 ATATATCTTG TGGAAAGGAC GACCTTTATG TCATGGCAGC ATGCGCACGC GTTAAGTCga 1381 caatcaacct ctggattaca aaatttgtga aagatt