Construct: ORF TRCN0000470184
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002102.1_s317c1
- Derived from:
- ccsbBroadEn_14158
- DNA Barcode:
- GACACCATACGCATCAGCTGTATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPR87 (53836)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470184
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 53836 | GPR87 | G protein-coupled receptor 87 | NM_023915.4 | 74.8% | 74.8% | (many diffs) |
| 2 | mouse | 84111 | Gpr87 | G protein-coupled receptor 87 | XM_006502394.3 | 67.4% | 72.7% | (many diffs) |
| 3 | mouse | 84111 | Gpr87 | G protein-coupled receptor 87 | NM_001302203.1 | 65.7% | 70.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 873
- ORF length:
- 807
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac gctgacattt ccatttcgaa tagtccatga tgcaggattt ggaccttggt 121 acttcaagtt tattctctgc agatacactt cagttttgtt ttatgcaaac atgtatactt 181 ccatcgtgtt ccttgggctg ataagcattg atcgctatct gaaggtggtc aagccatttg 241 gggactctcg gatgtacagc ataaccttca cgaaggtttt atctgtttgt gtttgggtga 301 tcatggctgt tttgtctttg ccaaacatca tcctgacaaa tggtcagcca acagaggaca 361 atatccatga ctgctcaaaa cttaaaagtc ctttgggggt caaatggcat acggcagtca 421 cctatgtgaa cagctgcttg tttgtggccg tgctggtgat tctgatcgga tgttacatag 481 ccatatccag gtacaTCCAC AAATCCAGCA GGCAATTCAT AAGTCAGTCA AGCCGAAAGC 541 GAAAACATAA CCAGAGCATC AGGGTTGTTG TGGCTGTGTT TTTTACCTGC TTTCTACCAT 601 ATCACTTGTG CAGAATTCCT TTTACTTTTA GTCACTTAGA CAGGCTTTTA GATGAATCTG 661 CACAAAAAAT CCTATATTAC TGCAAAGAAA TTACACTTTT CTTGTCTGCG TGTAATGTTT 721 GCCTGGATCC AATAATTTAC TTTTTCATGT GTAGGTCATT TTCAAGAAGG CTGTTCAAAA 781 AATCAAATAT CAGAACCAGG AGTGAAAGCA TCAGATCACT GCAAAGTGTG AGAAGATCGG 841 AAGTTCGCAT ATGTTATGAT TATACTGATG TGTACCCAAC TTTCTTGTAC AAAGTGGTTG 901 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 961 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGA 1021 CACCATACGC ATCAGCTGTA TCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1081 ttgtgaaaga tt