Construct: ORF TRCN0000470204
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017393.1_s317c1
- Derived from:
- ccsbBroadEn_12438
- DNA Barcode:
- TCATCAGCGCGCATCTGGCAGTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TP53AIP1 (63970)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470204
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 63970 | TP53AIP1 | tumor protein p53 regulated... | NM_001251964.1 | 99.6% | 99% | 20C>T |
| 2 | human | 63970 | TP53AIP1 | tumor protein p53 regulated... | NM_001195195.1 | 62.8% | 44.6% | (many diffs) |
| 3 | human | 63970 | TP53AIP1 | tumor protein p53 regulated... | XM_017018117.1 | 52.7% | 37.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 390
- ORF length:
- 324
- Sequence:
-
1 TCTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGGG ATCTTCCTCT GAGGTGAGCT TCAGATCTGC TCAAGCTTCC TGCAGTGGGG 121 CCAGGAGGCA GGGCCTGGGC AGGGGAGACC AGAACCTCTC GGTGATGCCT CCGAATGGCA 181 GGGCTCAGAC ACACACACCT GGCTGGGTAA GTCCCTGCAG TGAAAACCGA GACGGTCTTT 241 TGCCTGCCAC AGCCCCGGGC AGACTCTGCT CTCACCGTGG TGCCGACATC CCAAGTTTTC 301 AGACTCACCA GGACCCAGTG ACAGCATCTG GGTCCTCAGA GCTGCATGCG GACTGTCCCC 361 AGTTCAGAGC ATTGGACAGA GCTGGGAACT GCCCAACTTT CTTGTACAAA GTGGTTGATA 421 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 481 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATCATC 541 AGCGCGCATC TGGCAGTGCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 601 tgaaagatt