Construct: ORF TRCN0000470222

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010452.1_s317c1
Derived from:
ccsbBroadEn_07664
DNA Barcode:
GAGACATGGCCCCCTATTAAGAGA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SPINT2 (10653)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470222
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10653 SPINT2 serine peptidase inhibitor,... NM_021102.4 99.8% 99.6% 598G>C
2 human 10653 SPINT2 serine peptidase inhibitor,... NM_001166103.2 77.2% 76.9% 105_106ins171;427G>C
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
822
ORF length:
756
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc gcagctgtgc gggctgaggc ggagccgggc gtttctcgcc ctgctgggat
121cgctgctcct ctctggggtc ctggcggccg accgagaacg cagcatccac gacttctgcc
181tggtgtcgaa ggtggtgggc agatgccggg cctccatgcc taggtggtgg tacaatgtca
241ctgacggatc ctgccagctg tttgtgtatg ggggctgtga cggaaacagc aataattacc
301tgaccaagga ggagtgcctc aagaaatgtg ccactgtcac agagaatgcc acgggtgacc
361tggccaccag caggaatgca gcggattcct ctgtcccaag tgctcccaga aggcaggatt
421ctgaagacca ctccagcgat atgttcaact atgaagaata ctgcaccgcc aacgcagtca
481ctgGGCCTTG CCGTGCATCC TTCCCACGCT GGTACTTTGA CGTGGAGAGG AACTCCTGCA
541ATAACTTCAT CTATGGAGGC TGCCGGGGCA ATAAGAACAG CTACCGCTCT GAGGAGGCCT
601GCATGCTCCG CTGCTTCCGC CAGCAGGAGA ATCCTCCCCT GCCCCTTGGC TCAAAGGTGG
661TGCTTCTGGC GGGGCTGTTC GTGATGGTGT TGATCCTCTT CCTGGGAGCC TCCATGGTCT
721ACCTGATCCG GGTGGCACGG AGGAACCAGG AGCGTGCCCT GCGCACCGTC TGGAGCTCCG
781GAGATGACAA GGAGCAGCTG GTGAAGAACA CATATGTCCT GTGCCCAACT TTCTTGTACA
841AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
901AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
961AGGACGAGAG ACATGGCCCC CTATTAAGAG AACGCGTTAA GTCgacaatc aacctctgga
1021ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)