Construct: ORF TRCN0000470232
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006190.1_s317c1
- Derived from:
- ccsbBroadEn_14968
- DNA Barcode:
- TGCTTACCGCTGATTGTTAAACGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- P3H4 (10609)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470232
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10609 | P3H4 | prolyl 3-hydroxylase family... | NM_006455.3 | 99.9% | 99.7% | 1300C>T |
| 2 | human | 10609 | P3H4 | prolyl 3-hydroxylase family... | XM_024450538.1 | 82.7% | 82.5% | 1_273del;1573C>T |
| 3 | human | 10609 | P3H4 | prolyl 3-hydroxylase family... | XM_006721640.4 | 74.1% | 70.7% | (many diffs) |
| 4 | mouse | 66180 | P3h4 | prolyl 3-hydroxylase family... | NM_176830.2 | 86.9% | 90.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1377
- ORF length:
- 1311
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tcgggtggcg tgggggctgc tgtggttgct gctgggcagc gccggggcgc 121 agtacgagaa gtacagcttc cggggcttcc cgcccgagga cctgatgccg ctggccgcgg 181 cgtacgggca cgctctggag cagtacgagg gagagagctg gcgcgagagc gcgcgctacc 241 tggaggcggc gctgcggctg caccggctcc tgcgcgacag cgaggccttc tgccacgcca 301 actgcagcgg ccccgcgccc gcggccaagc ccgatcccga cggcggccgc gcagacgagt 361 gggcctgcga gctgcggctc ttcggccgcg tcctggagcg agccgcctgc ctgcggcgct 421 gcaagcggac gctgcccgcc ttccaggtgc cctacccgcc gcggcagctg ctgcgtgact 481 tccagagccg cctgccctac cagtacctgc actacgcgct gttcaaggct aaccggctgg 541 agaaggcggt ggcggcggcc tacaccttcc tccagaggaa cccgaagcac gagctgaccg 601 ccaagtatct caactactat caggggatgc tggacgtcgc cgacgagtcc ctcacggacc 661 tagaggccca gccctacgag gccgtgttcc tccgggctgt gaagctctac aacagcgggg 721 atttccgcag cagcacggag gacatggagc gggccttgtc agagtacctg gcagtctttg 781 cccggtgcct ggccggctgt gaaggggccc atgagcaggt ggacttcaag gacttctacc 841 cggccatagc agatctcttt gcagagtccc tgcagtgcaa ggtggactgt gaggccaatt 901 tgaCCCCCAA TGTGGGTGGC TACTTCGTGG ACAAGTTCGT GGCCACCATG TACCACTACC 961 TGCAGTTTGC CTACTATAAG TTGAATGATG TGCGCCAGGC TGCCCGCAGC GCCGCCAGCT 1021 ACATGCTCTT CGACCCCAAG GACAGCGTCA TGCAGCAGAA CCTGGTGTAT TACCGGTTCC 1081 ACCGGGCTCG CTGGGGCCTG GAAGAGGAGG ACTTCCAGCC CCGGGAGGAG GCCATGCTCT 1141 ACCACAACCA GACCGCCGAG CTGCGGGAGC TGCTGGAGTT CACCCACATG TACCTGCAGT 1201 CAGATGATGA GATGGAGCTG GAGGAGACAG AACCGCCCCT GGAGCCTGAG GATGCCCTAT 1261 CTGACGCCGA GTTTGAGGGG GAGGGTGACT ACGAGGAGGG CATGTATGCT GACTGGTGGC 1321 AGGAGCCGGA TGCCAAGGGT GACGAGGCCG AGGCTGAGCC AGAGTCTGAA CTCGCATGCC 1381 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1441 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1501 ATATATCTTG TGGAAAGGAC GATGCTTACC GCTGATTGTT AAACGAACGC GTTAAGTCga 1561 caatcaacct ctggattaca aaatttgtga aagatt