Construct: ORF TRCN0000470308
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014589.1_s317c1
- Derived from:
- ccsbBroadEn_12704
- DNA Barcode:
- CGTTCACCAGGGATGGCGTAGCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KHDC1 (80759)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470308
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80759 | KHDC1 | KH domain containing 1 | NM_030568.5 | 99.1% | 99.3% | 211_213delGTA;216C>T |
| 2 | human | 100129128 | KHDC1L | KH domain containing 1 like | NM_001126063.3 | 68.9% | 65.2% | (many diffs) |
| 3 | human | 80759 | KHDC1 | KH domain containing 1 | NM_001251874.2 | 68.6% | 68.7% | 1_219del;430_432delGTA;435C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 555
- ORF length:
- 489
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga catgggaacg agtgctcTCA GCAAGAAGCC GTGGTGGACC CTGCCTCAAA 121 ACTTTCATGC ACCAATGGTG TTTCACATGG AAGAGGACCA GGAGGAGCTC ATCTTCGGGC 181 ATGGTGACAC ATACCTTCGC TGCATTGAGG TGCACAGCCA CACCCTTATT CAGCTGGAGA 241 GTTGGTTCAC AGCTACAGGC CAGACTCGTG TGACTGTTGG ACCACACAGG GCAAGGCAGT 301 GGCTGCTGCA CATGTTTTGT TGTGTGGGGA GCCAGGACTC CTATCATCAT GCTCGAGGCC 361 TGGAGATGCT GGAACGTGTC CGAAGCCAGC CTCTGACCAA CGATGACCTG GTCACCTCCA 421 TTAGTGTGCC ACCATACACT GGAGACCTGT CTTTGGCTCC CAGGATAAGT GGAACCGTGT 481 GTCTTAGCGT TCCTCAGCCT TCCCCTTACC AAGTGATTGG TTGTTCGGGT TTCCATTTGA 541 GTTCACTGTA TCCGTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 601 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 661 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CGTTCACCAG GGATGGCGTA 721 GCACACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt