Construct: ORF TRCN0000470348
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010346.1_s317c1
- Derived from:
- ccsbBroadEn_09165
- DNA Barcode:
- CCCCAGCAGTGTGAGCGCGGTTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SYT3 (84258)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470348
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84258 | SYT3 | synaptotagmin 3 | NM_001160328.1 | 99.7% | 99.8% | (many diffs) |
| 2 | human | 84258 | SYT3 | synaptotagmin 3 | NM_001160329.2 | 99.7% | 99.8% | (many diffs) |
| 3 | human | 84258 | SYT3 | synaptotagmin 3 | NM_032298.2 | 99.7% | 99.8% | (many diffs) |
| 4 | mouse | 20981 | Syt3 | synaptotagmin III | NM_001114116.1 | 87.1% | 94.9% | (many diffs) |
| 5 | mouse | 20981 | Syt3 | synaptotagmin III | NM_016663.3 | 87.1% | 94.9% | (many diffs) |
| 6 | mouse | 20981 | Syt3 | synaptotagmin III | XM_006540755.2 | 87.1% | 94.9% | (many diffs) |
| 7 | mouse | 20981 | Syt3 | synaptotagmin III | XM_017322087.1 | 87.1% | 94.9% | (many diffs) |
| 8 | mouse | 20981 | Syt3 | synaptotagmin III | XM_017322088.1 | 87.1% | 94.9% | (many diffs) |
| 9 | mouse | 20981 | Syt3 | synaptotagmin III | XM_017322089.1 | 87.1% | 94.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1836
- ORF length:
- 1770
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc aggagactat gaggatgacc tctgccggcg ggcactcatc ctggtctcgg 121 acctctgtgc gcgggtccga gatgctgaca ccaacgacag gtgccaggag ttcaatgacc 181 gaatccgagg ctatccccgg ggtccagatg cagacatctc cgtgagcctg ctgtcggtca 241 tcgtgacatt ctgtggcatt gtccttctgg gtgtctctct cttcgtgtcc tggaagttgt 301 gctgggtgca ctggcgggac aagggaggct cggcagtggg cggtggcccc ctgcgcaaag 361 acctaggccc tggtgtcggg ctggcaggcc tggtaggcgg aggcgggcac cacctggcgg 421 ctggcctggg tggccatcct ctgctgggcg gcccacacca ccatgcccat gccgcccacc 481 atccaccctt tgctgagctg ctggagccag gcagcctggg gggttctgac acccctgagc 541 cctcctactt ggacatggac tcgtatccag aggctgcagc agcagcagtg gccgctgggg 601 tcaaaccgag ccaaacatcc cctgagctgc cctctgaggg gggagcaggc tctgggttgc 661 tcctgctgcc ccccagtggt gggggcttgc ccagtgccca gtcacatcag caggtcacaa 721 gcctggcacc cactaccagg tacccagccc tgccccgacc cctcacccag cagactctga 781 cctcccagcc ggaccccagc agtgaggagc gcccacctgc cctgccctta cccctgcctg 841 gaggcgagga aaaagccaaa ctcattgggc agattaagcc agagctgtac caggggactg 901 gccctggtgg ccggcggagc ggtgggggcc caggctctgg agaggcaggc acaggggcac 961 cctgtggccg tatcagcttc gccctgcggt acctctatgg ctcggaccag ctggtggtga 1021 ggatcctgca ggccctggac ctccctgcca aggactccaa cggcttctca gacccctacg 1081 tcaagatcta cctgctgcct gaccgcaaga aaaagtttca gaccaaggtg cacaggaaga 1141 ccctgaaccc cgtcttcaat gagacgtttc aattctcggt gcccctggcc gagctggccc 1201 aacgcaaact gcacttcagc gtctatgact ttgaccgctt ctcgcggcac gacctcatcg 1261 gccaggtggt gctggacaac ctcctggagc tggccgagca gccccctgac cgcccgctct 1321 ggagggacat cgtggagggc ggctcggaaa aagcagatct tggggagctc aacttctcac 1381 tctgctacct ccccacggcc gGGCGCCTCA CCGTGACCAT CATCAAAGCC TCTAACCTCA 1441 AAGCGATGGA CCTCACTGGC TTCTCAGACC CCTACGTGAA GGCCTCCCTG ATCAGCGAGG 1501 GGCGGCGTCT GAAGAAGCGG AAAACCTCCA TCAAGAAGAA CACGCTGAAC CCCACCTATA 1561 ATGAGGCGCT GGTGTTCGAC GTGGCCCCCG AGAGCGTGGA GAACGTGGGG CTCAGCATCG 1621 CCGTGGTGGA CTACGACTGC ATCGGGCACA ACGAGGTGAT CGGCGTGTGC CGTGTGGGCC 1681 CCGACGCTGC CGACCCGCAC GGCCGCGAGC ACTGGGCAGA GATGCTGGCC AATCCCCGCA 1741 AGCCCGTGGA GCACTGGCAT CAGCTAGTGG AGGAAAAGAC TGTGACCAGC TTCACAAAAG 1801 GCAGCAAAGG ACTATCAGAG AAAGAGAACT CCGAGTGCCC AACTTTCTTG TACAAAGTGG 1861 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1921 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1981 ACCCCAGCAG TGTGAGCGCG GTTCTACGCG TTAAGTCgac aatcaacctc tggattacaa 2041 aatttgtgaa agatt