Construct: ORF TRCN0000470363
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017266.1_s317c1
- Derived from:
- ccsbBroadEn_10596
- DNA Barcode:
- GGTATGAGGGCGGGTCGCCTCCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FBXL19-AS1 (283932)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470363
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 283932 | FBXL19-AS1 | FBXL19 antisense RNA 1 | NR_024348.1 | 9.2% | 1_305del;516C>T;672_3951del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 435
- ORF length:
- 366
- Sequence:
-
1 tcttccattt CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCACCAT GGCGGAACCA GGAGGACGAG GAGACTACCG TAAAGATGGC AGGCTACCTA 121 GCCTCTCCCG GTCTCCGCTT TCCACCACCT TGGGCACCTC GCCCGCCTGT GGTCTCGAAA 181 TCCCCCCCAC CAGTGGCGCA CGGCCTGACG GGAGTTGCAG TCTCCCGGCT CCCGTCTATC 241 ATCTCAAGTC GAGACAATGG AAGGGGATGG GGCGGGGCTA CCGGCAAAGA TGGCGGCTGC 301 AGGGCCGGGG CGACTGCGAC ATGGGATGTG TAGTCCAGGC GTCTGGCCTC TTCCCTGCGG 361 AAATGAGGGA GAGGACTACG AAAAAGATGG CGACCTCGCC CCTCGACCTC TGCGCCGGTG 421 CCTGCTGGGA AATGTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 481 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 541 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GGTATGAGGG CGGGTCGCCT 601 CCGTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt