Construct: ORF TRCN0000470385
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006374.1_s317c1
- Derived from:
- ccsbBroadEn_12642
- DNA Barcode:
- GGGCCGCCCCTACCTTAACAGGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- THNSL1 (79896)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470385
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79896 | THNSL1 | threonine synthase like 1 | NM_024838.5 | 29.8% | 29.8% | 1_1563del |
| 2 | human | 79896 | THNSL1 | threonine synthase like 1 | XM_005252597.3 | 29.8% | 29.8% | 1_1563del |
| 3 | human | 79896 | THNSL1 | threonine synthase like 1 | XM_017016665.1 | 29.8% | 29.8% | 1_1563del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 732
- ORF length:
- 666
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat gggaatcccg attcgaaaat ttatctgtgc ctctaatcag aaccatgttt 121 tgactgattt tataaaaaca ggacattatg atctaaggga aagaaaacta gcacaaacct 181 tttcaccgtc aatagatatt ctcaaatctt caaacctaga acgacattta cacttgatgg 241 ctaataaaga tggacagcta atgacagaat tatttaatcg attagaaagt cagcatcatt 301 tccagataga aaaGGCTCTA GTTGAGAAAC TTCAGCAGGA TTTTGTAGCT GACTGGTGCT 361 CTGAGGGAGA GTGCCTAGCA GCTATTAACT CCACCTATAA TACTTCAGGG TATATTTTGG 421 ATCCACACAC TGCTGTTGCA AAAGTGGTTG CAGATAGGGT GCAAGACAAA ACTTGCCCTG 481 TGATTATCTC ATCTACAGCC CATTACTCAA AGTTTGCACC TGCTATCATG CAGGCTTTAA 541 AGATTAAAGA AATCAATGAG ACTTCATCAA GTCAGCTCTA TTTGCTGGGT TCATACAATG 601 CATTACCTCC ACTGCATGAG GCTTTATTAG AGAGAACAAA ACAGCAAGAG AAGATGGAGT 661 ACCAGGTCTG TGCAGCTGAT ATGAATGTCT TGAAGAGTCA TGTGGAACAA CTTGTCCAAA 721 ATCAATTCAT ATGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 781 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 841 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGGG CCGCCCCTAC CTTAACAGGG 901 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t