Construct: ORF TRCN0000470412
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016509.1_s317c1
- Derived from:
- ccsbBroadEn_09091
- DNA Barcode:
- TAAATGCGTGTGTACCCCCATGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLIRP (81892)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470412
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81892 | SLIRP | SRA stem-loop interacting R... | NM_031210.6 | 99.6% | 100% | 19A>C |
| 2 | human | 81892 | SLIRP | SRA stem-loop interacting R... | NM_001267863.1 | 97.8% | 98.1% | 19A>C;262_263insAGGTCC |
| 3 | human | 81892 | SLIRP | SRA stem-loop interacting R... | NM_001267864.1 | 82.9% | 80.7% | (many diffs) |
| 4 | human | 81892 | SLIRP | SRA stem-loop interacting R... | NR_052025.2 | 66.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 393
- ORF length:
- 327
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcctcagca gcgcgaggtg ctgcggcgct gcgtagaagt atcaatcagc 121 cggttgcttt tgtgagaaga attccttgga cTGCGGCGTC GAGTCAGCTG AAAGAACACT 181 TTGCACAGTT CGGCCATGTC AGAAGGTGCA TTTTACCTTT TGACAAGGAG ACTGGCTTTC 241 ACAGAGGTTT GGGTTGGGTT CAGTTTTCTT CAGAAGAAGG ACTTCGGAAT GCACTACAAC 301 AGGAAAATCA TATTATAGAT GGAGTAAAGG TCCAGGTTCA CACTAGAAGG CCAAAACTTC 361 CGCAAACATC TGATGATGAA AAGAAAGATT TTTGCCCAAC TTTCTTGTAC AAAGTGGTTG 421 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 481 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATA 541 AATGCGTGTG TACCCCCATG TCACGCGTTA AGTCgacaat caacctctgg attacaaaat 601 ttgtgaaaga tt