Construct: ORF TRCN0000470441
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015878.1_s317c1
- Derived from:
- ccsbBroadEn_11369
- DNA Barcode:
- TTCTTCAACGTTCCTACGGTCCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HAND2 (9464)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470441
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9464 | HAND2 | heart and neural crest deri... | NM_021973.3 | 82.9% | 82.9% | 64_81del;211_303del |
| 2 | mouse | 15111 | Hand2 | heart and neural crest deri... | NM_010402.4 | 78.8% | 82.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 609
- ORF length:
- 540
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagtctggta ggtggttttc cccaccaccc ggtggtgcac cacgagggct 121 acccgtttgc cgccgccgcc gccgccagcc gctgcagcca tgaggagaac ccctacttcc 181 atggctggcT CATCGGCCAC CCCGAGATGT CGCCCCCCGA CTACAGCATG GCCCTGTCCT 241 ACAGCCCCGA GTATGCCAGC GGCACCGCCA ACCGCAAGGA GCGGCGCAGG ACTCAGAGCA 301 TCAACAGCGC CTTCGCCGAA CTGCGCGAGT GCATCCCCAA CGTACCCGCC GACACCAAAC 361 TCTCCAAAAT CAAGACCCTG CGCCTGGCCA CCAGCTACAT CGCCTACCTC ATGGACCTGC 421 TGGCCAAGGA CGACCAGAAT GGCGAGGCGG AGGCCTTCAA GGCAGAGATC AAGAAGACCG 481 ACGTGAAAGA GGAGAAGAGG AAGAAGGAGC TGAACGAAAT CTTGAAAAGC ACAGTGAGCA 541 GCAACGACAA GAAAACCAAA GGCCGGACGG GCTGGCCGCA GCACGTCTGG GCCCTGGAGC 601 TCAAGCAGTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 661 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 721 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATTCTTC AACGTTCCTA CGGTCCTTAC 781 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt