Construct: ORF TRCN0000470525
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001036.1_s317c1
- Derived from:
- ccsbBroadEn_12229
- DNA Barcode:
- CCTGTATTCGTTGACCTCGTTGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNPC3 (55599)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470525
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55599 | RNPC3 | RNA binding region (RNP1, R... | NM_017619.4 | 50.4% | 50.4% | 1_768del |
| 2 | mouse | 67225 | Rnpc3 | RNA-binding region (RNP1, R... | NM_001038696.1 | 43.9% | 43.5% | (many diffs) |
| 3 | mouse | 67225 | Rnpc3 | RNA-binding region (RNP1, R... | NM_026043.3 | 43.9% | 43.5% | (many diffs) |
| 4 | mouse | 67225 | Rnpc3 | RNA-binding region (RNP1, R... | NM_001287015.1 | 43.7% | 43.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 849
- ORF length:
- 783
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa caaattaatg gaactagcaa atcttcagcc caaaagacct aaaacaataa 121 agcagcgcca tgtgagaaaa aagagaaaaa taaaggatat gttgaataca cctttgtgtc 181 cttcacacag cagtttacat ccagtgctgt taccttcaga tgtatttgac caaccacaac 241 ctgtaggtaa caaaagaatt gaattccata tatctaccga catgccagct gcatttaaga 301 aagatttaga aaaggaacaa aattgtgagg aaaaaaatca tgatttacct gctactgaag 361 ttgatgcatc caatatagga tttggaaaaa tcttccccaa acctaatttG GACATCACAG 421 AGGAGATTAA AGAAGACTCT GATGAAATGC CTTCAGAATG TATTTCTAGA AGGGAATTGG 481 AAAAGGGCAG AATTTCTAGA GAAGAAATGG AAACACTTTC AGTTTTCAGA AGTTATGAAC 541 CGGGTGAACC AAACTGTAGA ATTTATGTAA AGAATTTAGC TAAACATGTT CAAGAAAAGG 601 ACCTTAAATA TATTTTTGGA AGATATGTTG ACTTTTCATC AGAAACACAG CGGATCATGT 661 TTGATATACG TTTGATGAAA GAAGGTCGTA TGAAAGGACA AGCTTTCATT GGACTTCCTA 721 ATGAAAAAGC AGCAGCAAAA GCCTTAAAGG AAGCTAATGG ATATGTGCTT TTTGGAAAAC 781 CCATGGTGGT TCAGTTTGCT CGATCTGCTA GACCAAAACA AGATCCTAAG GAAGGAAAAA 841 GAAAGTGTTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 901 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 961 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACCTGTA TTCGTTGACC TCGTTGTCAC 1021 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt