Construct: ORF TRCN0000470546
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008180.1_s317c1
- Derived from:
- ccsbBroadEn_05278
- DNA Barcode:
- AACTAGGTGAATTCACTATTAGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DEFB119 (245932)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470546
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 245932 | DEFB119 | defensin beta 119 | NM_153289.4 | 100% | 100% | |
| 2 | human | 245932 | DEFB119 | defensin beta 119 | NR_073153.1 | 45.4% | 1_166del;225_264del;459_555del | |
| 3 | human | 245932 | DEFB119 | defensin beta 119 | NR_073151.1 | 42.5% | 1_166del;225_301del;496_592del | |
| 4 | human | 245932 | DEFB119 | defensin beta 119 | NR_073152.1 | 37.6% | (many diffs) | |
| 5 | human | 245932 | DEFB119 | defensin beta 119 | NR_126440.2 | 36.9% | 1_107del;165_166ins88;272_356del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 342
- ORF length:
- 252
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGAAACTTCT TTACCTGTTT CTTGCCATCC 121 TTCTGGCCAT AGAAGAACCA GTGATATCAG GCAAACGCCA CATCCTTCGA TGCATGGGTA 181 ACAGTGGAAT TTGTAGGGCC TCTTGCAAAA AGAACGAACA GCCCTACCTC TATTGCAGAA 241 ATTGTCAGTC CTGCTGCCTC CAGTCCTACA TGAGGATAAG CATTTCTGGC AAAGAGGAAA 301 ATACCGACTG GTCTTATGAG AAGCAGTGGC CAAGACTACC TTGCCCAACT TTCTTGTACA 361 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 421 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 481 AGGACGAAAC TAGGTGAATT CACTATTAGC CACGCGTTAA GTCgacaatc aacctctgga 541 ttacaaaatt tgtgaaagat t