Construct: ORF TRCN0000470616
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007493.3_s317c1
- Derived from:
- ccsbBroadEn_11495
- DNA Barcode:
- CTTCTCCACTTAGCTGCTGATTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TESK2 (10420)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470616
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10420 | TESK2 | testis associated actin rem... | NM_007170.3 | 94.8% | 94.7% | 793_879del;1363C>T |
| 2 | human | 10420 | TESK2 | testis associated actin rem... | NM_001320800.2 | 80.3% | 80.2% | 0_1ins249;544_630del;1114C>T |
| 3 | mouse | 230661 | Tesk2 | testis-specific kinase 2 | NM_146151.4 | 82.2% | 82.8% | (many diffs) |
| 4 | mouse | 230661 | Tesk2 | testis-specific kinase 2 | XM_006503008.3 | 64.4% | 64.2% | (many diffs) |
| 5 | mouse | 230661 | Tesk2 | testis-specific kinase 2 | XR_001784124.1 | 42% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1692
- ORF length:
- 1626
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tcggagcaaa cggaattcaa ttgcaggatt tcctccacgt gtggagcgtc 121 ttgaagagtt tgaaggaggt ggtggaggag aaggaaatgt gagccaggtg ggaagagttt 181 ggccatcttc gtatcgagct cttataagtg ccttttccag actgacgcgt ttggatgatt 241 tcacctgtga aaaaataggg tctggcttct tttctgaagt gttcaaggta cgacaccgag 301 cttctggtca ggtgatggct cttaagatga acacattgag cagtaaccgg gcaaacatgc 361 tgaaagaagt acagctcatg aatagactct cccatcccaa catccttagg ttcatgggtg 421 tatgtgttca tcaaggacaa ttgcatgcac ttacagagta tatcaactcc gggaacctgg 481 aacagttgct agacagtaac ctgcatttgc cttggactgt gagggtaaaa ctggcctatg 541 acatagcagt gggcctcagc taccttcact tcaaaggcat ttttcatcgg gacctcacat 601 ctaagaactg cctgataaag agggatgaga atggttactc tgcagtggta gctgactttg 661 gcctggctga gaagatcccc gatgtcagca tggggagtga gaagctggcc gtggtgggtt 721 ccccattctg gatggcacct gaggttctcc gagatgagcc ctataatgaa aaggcagatg 781 tgttctctta tggtatcatc ctctgcgaga tcatcgcccg catccaggcc gatccggact 841 atcttccccg cacagagatg gatcccaaac tgcgcccatc ttttgtggag attgggaaga 901 ccctggagga aattctgagc cgcctacagg aagaagagca ggagagggat aggaagctgc 961 agcccacagc caggggactc ttggagaaag cacctggggt gaagcgacta agctcactgg 1021 atgacaagat cccccacaag tcaccatgcc caagacgtac catctggctg tctcgaagcc 1081 agtcagatat cttttcccgt aagcccccac gtacagtgag tgtcttggac ccatactacc 1141 ggccacgaga tggtgctgcc cgcaccccca aagtcaaccc ttttagtgct cgccaggacc 1201 tcatgggggg caagatcaag ttttttgacc tgcccagcaa gtctgtcatc tctctggtat 1261 ttgacctGGA TGCACCAGGG CCCGGAACTA TGCCCCTGGC TGACTGGCAG GAGCCCCTGG 1321 CCCCACCTAT TCGCCGGTGG TGTTCCTTGC CTGGTTCGCC TGAGTTCTTG CATCAAGAGG 1381 CTTGTCCATT TGTGGGCCGG GAAGAATCGC TATCTGATGG GCCCCCACCA CGCCTAAGTA 1441 GTCTCAAGTA CAGAGTTAAA GAGATCCCAC CATTCCGGGC ATCTGCCCTA CCAGCTGCTC 1501 AAGCCCATGA GGCTATGGAC TGCTCCATTC TCCAGGAAGA AAATGGTTTT GGGTCCAGGC 1561 CCCAGGGGAC CAGTCCATGC CCTGCGGGTG CTTCTGAGGA GATGGAGGTA GAAGAAAGGC 1621 CAGCAGGCTC AACTCCAGCC ACCTTCTCCA CCTCAGGCAT AGGCCTGCAA ACCCAGGGAA 1681 AGCAGGATGG GTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1741 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1801 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACTT CTCCACTTAG CTGCTGATTC 1861 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t