Construct: ORF TRCN0000470619
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010791.1_s317c1
- Derived from:
- ccsbBroadEn_13790
- DNA Barcode:
- GCACATTCACGCTCTTGCGAGTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470619
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100423062 | IGLL5 | immunoglobulin lambda like ... | NM_001256296.2 | 52.8% | 43.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 774
- ORF length:
- 708
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cggcttccct ctcctcctca ccctcctcac tcactgtgca gggtcctggg 121 cccaatctgt gctgactcag ccaccctcag cgtctgggtc ccccgggcag agggtcacca 181 tctcttgttc tggaagcagg tccaacatcg gaagtaatta tgtatactgg taccagcaag 241 tcccaggaac ggcccccaaa ctcctcatct ataggaatga tcagcggccc tcaggggtcc 301 ctgaccgatt ctctggctcc aagtctggca cctcagcctc cctggccatc agtgggctcc 361 ggtccgagga tgaggctgat tattactgtg cagcatggga tgacagcctg agtgtccatg 421 tggtattcgg cggagggacc aaactgaccg tcctcagtca gcccaaggct gccccctcgg 481 tcactctgtt cccaccctcc tctgaggagc ttcaagccaa caaggccaca cTGGTGTGTC 541 TCATAAGTGA CTTCTACCCG GGAGCCGTGA CAGTGGCCTG GAAGGCAGAT AGCAGCCCCG 601 TCAAGGCGGG AGTGGAGACC ACCACACCCT CCAAACAAAG CAACAACAAG TACGCGGCCA 661 GCAGCTACCT GAGCCTGACG CCTGAGCAGT GGAAGTCCCA CAAAAGCTAC AGCTGCCAGG 721 TCACGCATGA AGGGAGCACC GTGGAGAAGA CAGTGGCCCC TACAGAATGT TCATACCCAA 781 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 841 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 901 TATCTTGTGG AAAGGACGAG CACATTCACG CTCTTGCGAG TAAACGCGTT AAGTCgacaa 961 tcaacctctg gattacaaaa tttgtgaaag att