Construct: ORF TRCN0000470752
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008166.1_s317c1
- Derived from:
- ccsbBroadEn_03324
- DNA Barcode:
- GAATACTCTCGTCTGTTGGCCTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM14C (51522)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470752
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51522 | TMEM14C | transmembrane protein 14C | NM_001165258.1 | 100% | 100% | |
| 2 | human | 51522 | TMEM14C | transmembrane protein 14C | NM_016462.4 | 100% | 100% | |
| 3 | human | 81853 | TMEM14B | transmembrane protein 14B | NM_030969.5 | 88% | 73.6% | (many diffs) |
| 4 | human | 81853 | TMEM14B | transmembrane protein 14B | NM_001286484.1 | 73% | 52.4% | (many diffs) |
| 5 | human | 81853 | TMEM14B | transmembrane protein 14B | NM_001286488.1 | 64.1% | 52.3% | (many diffs) |
| 6 | human | 81853 | TMEM14B | transmembrane protein 14B | NM_001127711.2 | 60.4% | 46% | (many diffs) |
| 7 | mouse | 66154 | Tmem14c | transmembrane protein 14C | XM_006516938.3 | 77.8% | 82.3% | (many diffs) |
| 8 | mouse | 66154 | Tmem14c | transmembrane protein 14C | XM_006516939.2 | 77.8% | 82.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 67
- ORF end:
- 403
- ORF length:
- 336
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttcggcatgc aggacactgg ctcagtagtg cctttgcatt ggtttggctt tGGCTACGCA 121 GCACTGGTTG CTTCTGGTGG GATCATTGGC TATGTAAAAG CAGGCAGCGT GCCGTCCCTG 181 GCTGCAGGGC TGCTCTTTGG CAGTCTAGCC GGCCTGGGTG CTTACCAGCT GTCTCAGGAT 241 CCAAGGAACG TTTGGGTTTT CCTAGCTACA TCTGGTACCT TGGCTGGCAT TATGGGAATG 301 AGGTTCTACC ACTCTGGAAA ATTCATGCCT GCAGGTTTAA TTGCAGGTGC CAGTTTGCTG 361 ATGGTCGCCA AAGTTGGAGT TAGTATGTTC AACAGACCCC ATTACCCAAC TTTCTTGTAC 421 AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG 481 TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA 541 AAGGACGAGA ATACTCTCGT CTGTTGGCCT CGACGCGTTA AGTCgacaat caacctctgg 601 attacaaaat ttgtgaaaga tt