Construct: ORF TRCN0000470754
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014557.1_s317c1
- Derived from:
- ccsbBroadEn_12104
- DNA Barcode:
- CTGATTGGGGCAGAAAACGAGTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NSUN2 (54888)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470754
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54888 | NSUN2 | NOP2/Sun RNA methyltransfer... | NM_001193455.2 | 77.3% | 77.3% | 1_498del |
| 2 | human | 54888 | NSUN2 | NOP2/Sun RNA methyltransfer... | NM_017755.6 | 73.7% | 73.7% | 1_603del |
| 3 | human | 54888 | NSUN2 | NOP2/Sun RNA methyltransfer... | NR_037947.2 | 56.9% | (many diffs) | |
| 4 | mouse | 28114 | Nsun2 | NOL1/NOP2/Sun domain family... | NM_145354.5 | 60.9% | 63.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1764
- ORF length:
- 1698
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa tgtccccttt ccagagggat ttgttattgc gaatgatgtg gacaacaagc 121 gctgctacct gctcgtccat caagccaaga ggctgagcag cccctgcatc atggtggtca 181 accatgatgc ctccagcata cccaggctcc agatagatgt ggacggcagg aaagagatcc 241 tcttctatga tcgaatttta tgtgatgtcc cttgcagtgg agacggcact atgagaaaaa 301 acattgatgt ttggaaaaag tggaccacct taaatagctt gcagctacat ggcttacagc 361 tgcggattgc aacacgcggg gctgaacagc tggctgaagg tggaaggatg gtgtattcca 421 cgtgttcact aaaccctatt gaggatgaag cagtcatagc atctttactg gaaaaaagtg 481 aaggtgcttt ggagcttgct gatgtgtcta atgaactgcc agggctgaag tggatgcctg 541 gaatcacaca gtggaaggta atgacgaaag atgggcagtg gtttacagac tgggacgctg 601 ttcctcacag cagacacacc cagatccgac ctaccatgtt ccctccgaag gacccagaaa 661 agctgcaggc catgcacctg gagcgatgcc ttaggatatt accccatcat cagaatactg 721 gagggttttt tgtggcagta ttggtgaaaa aatcttcaat gccgtggaat aaacgtcagc 781 caaagcttca gggtaaatct gcagagacca gagaaagcac acagctgagc cctgcagatc 841 tcacagaagg gaaacccaca gatccctcta agctggaaag tccgtcattc acaggaactg 901 gtgacacaga aatagctcat gcaactgagg atttagagaa taatggcagt aagaaagatg 961 gcgtgtgtgg tcctcctcca tcaaagaaaa tgaagttatt tggatttaaa gaagatccat 1021 ttgtatttat tcctgaagat gacccattat ttccacctat tgagaaattt tatgctttgg 1081 atccttcatt cccaaggatg aatttgttaa ctcggactac agaagggaag aaaaggcagc 1141 tctacatggt ttctaaggag ttgcggaatg tgctgctgaa taacagtgag aagatgaagg 1201 ttattaacac ggggatcaaa gtctggtgta gaaataacag cggtgaagag tttgactgtg 1261 ctttccggct ggcacaggag ggaatatata cattgtatcc atttattaac tcaagaatta 1321 ttactgtatc aatggaagat gttaagatac tgttgaccca ggaaaatccc ttttttagaa 1381 aactcagcag tgagaccTAC AGTCAAGCAA AGGACCTGGC AAAGGGAAGC ATCGTGCTGA 1441 AGTATGAACC AGATTCTGCG AATCCAGACG CTCTGCAGTG TCCCATCGTC TTATGCGGAT 1501 GGCGGGGAAA GGCCTCCATT CGAACTTTTG TGCCCAAGAA TGAACGGCTT CATTATCTCA 1561 GGATGATGGG GCTGGAGGTA TTGGGAGAAA AGAAGAAGGA AGGGGTTATC CTCACAAATG 1621 AGAGTGCAGC CAGCACCGGA CAGCCAGACA ATGACGTGAC TGAGGGACAG AGAGCAGGAG 1681 AGCCCAACAG CCCAGATGCA GAAGAGGCCA ACAGTCCAGA CGTGACAGCA GGCTGTGACC 1741 CGGCGGGGGT CCATCCACCC CGGTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1801 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1861 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC TGATTGGGGC 1921 AGAAAACGAG TCAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1981 att