Construct: ORF TRCN0000470762
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008460.1_s317c1
- Derived from:
- ccsbBroadEn_00941
- DNA Barcode:
- AACCACACCGCCAAAATCAGCCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LIG3 (3980)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470762
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3980 | LIG3 | DNA ligase 3 | NM_013975.4 | 100% | 100% | |
| 2 | human | 3980 | LIG3 | DNA ligase 3 | XM_017024624.1 | 100% | 100% | |
| 3 | human | 3980 | LIG3 | DNA ligase 3 | XM_005257970.4 | 99.1% | 99.1% | 1_27del |
| 4 | human | 3980 | LIG3 | DNA ligase 3 | NM_002311.4 | 93.7% | 92.3% | (many diffs) |
| 5 | human | 3980 | LIG3 | DNA ligase 3 | XM_006721896.3 | 92.9% | 91.5% | (many diffs) |
| 6 | mouse | 16882 | Lig3 | ligase III, DNA, ATP-dependent | NM_001291246.1 | 87.6% | 88.2% | (many diffs) |
| 7 | mouse | 16882 | Lig3 | ligase III, DNA, ATP-dependent | NM_001291247.1 | 87.6% | 88.2% | (many diffs) |
| 8 | mouse | 16882 | Lig3 | ligase III, DNA, ATP-dependent | NM_001291245.1 | 87.6% | 88.1% | (many diffs) |
| 9 | mouse | 16882 | Lig3 | ligase III, DNA, ATP-dependent | NM_010716.3 | 87.6% | 88.1% | (many diffs) |
| 10 | mouse | 16882 | Lig3 | ligase III, DNA, ATP-dependent | XM_006532350.3 | 87.6% | 88.1% | (many diffs) |
| 11 | mouse | 16882 | Lig3 | ligase III, DNA, ATP-dependent | XM_006532348.3 | 83.2% | 83.7% | (many diffs) |
| 12 | mouse | 16882 | Lig3 | ligase III, DNA, ATP-dependent | XM_006532347.3 | 83.1% | 83.6% | (many diffs) |
| 13 | mouse | 16882 | Lig3 | ligase III, DNA, ATP-dependent | XM_006532353.3 | 78.1% | 77% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3093
- ORF length:
- 3027
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tttggctttc aagatcttct ttccacaaac cctccgtgca ctcagccgaa 121 aagaactgtg cctattccga aaacatcact ggcgtgatgt aagacaattc agccagtggt 181 cagaaacaga tctgcttcat ggacatcccc tcttcctgag aagaaagcct gttctatcat 241 tccagggaag ccatctaaga tcacgtgcca cctaccttgt tttcttgcca gggttgcatg 301 tgggactctg cagtggcccc tgtgagatgg ctgagcaacg gttctgtgtg gactatgcca 361 agcgtggcac agctggctgc aaaaaatgca aggaaaagat tgtgaagggc gtatgccgaa 421 ttggcaaagt ggtgcccaat cccttctcag agtctggggg tgatatgaaa gagtggtacc 481 acattaaatg catgtttgag aaactagagc gggcccgggc caccacaaaa aaaatcgagg 541 acctcacaga gctggaaggc tgggaagagc tggaagataa tgagaaggaa cagataaccc 601 agcacattgc agatctgtct tctaaggcag caggtacacc aaagaagaaa gctgttgtcc 661 aggctaagtt gacaaccact ggccaggtga cttctccagt gaaaggcgcc tcatttgtca 721 ccagtaccaa tccccggaaa ttttctggct tttcagccaa gcccaacaac tctggggaag 781 ccccctcgag ccccacccct aagagaagtc tgtcttcaag caaatgtgac cccaggcata 841 aggactgtct gctacgggag tttcgaaagt tatgcgccat ggtggccgat aatcctagct 901 acaacacgaa gacccagatc atccaggact tccttcggaa aggctcagca ggagatggtt 961 tccacggtga tgtgtaccta acagtgaagc tgctgctgcc aggagtcatt aagactgttt 1021 acaacttgaa cgataagcag attgtgaagc ttttcagtcg catttttaac tgcaacccag 1081 atgatatggc acgggaccta gagcagggtg acgtgtcaga gacaatcaga gtcttctttg 1141 agcagagcaa gtctttcccc ccagctgcca agagcctcct taccatccag gaagtggatg 1201 agttccttct gcggctgtcc aagctcacca aggaggatga gcagcaacag gccctacagg 1261 acattgcctc caggtgtaca gccaatgacc ttaaatgcat catcaggttg atcaaacatg 1321 atctgaagat gaactcaggt gcaaaacatg tgttagacgc ccttgacccc aatgcctatg 1381 aagccttcaa agcctcgcgc aacctgcagg atgtggtgga gcgggtcctt cacaacgcgc 1441 aggaggtgga gaaggagccg ggccagagac gagctctgag cgtccaggcc tcgctgatga 1501 cacctgtgca gcccatgttg gcggaggcct gcaagtccgt tgagtatgca atgaagaaat 1561 gtcccaatgg catgttctct gagatcaagt acgatggaga gcgagtccag gtgcataaga 1621 atggagacca cttcagctac ttcagccgca gtctcaagcc cgtccttcct cacaaggtgg 1681 cccactttaa ggactacatt ccccaggctt ttcctggggg ccacagcatg atcttggatt 1741 ctgaagtgct tctgattgac aacaagacag gcaaaccact gccctttggg actctgggag 1801 tacacaagaa agcagccttc caggatgcta atgtctgcct gtttgttttt gattgtatct 1861 actttaatga tgtcagcttg atggacagac ctctgtgtga gcggcggaag tttcttcatg 1921 acaacatggt tgaaattcca aaccggatca tgttctcaga aatgaagcga gtcacaaaag 1981 ctttggactt ggctgacatg ataacccggg tgatccagga gggattggag gggctggtgc 2041 tgaaggatgt gaagggtaca tatgagcctg ggaagcggca ctggctgaaa gtgaagaaag 2101 actatttgaa cgagggggcc atggccgaca cagctgacct ggtggtcctt ggagccttct 2161 atgggcaagg gagcaaaggc ggcatgatgt caatcttcct catgggctgc tacgaccctg 2221 gcagccagaa gtggtgcaca gtcaccaagt gtgcaggagg ccatgatgat gccacgcttg 2281 cccgcctgca gaatgaacta gacatggtga agatcagcaa ggaccccagc aaaataccca 2341 gctggttgaa ggtcaacaag atctactatc ctgacttcat cgtcccagac ccaaagaaag 2401 ctgccgtgtg ggagatcaca ggggctgaat tctccaaatc ggaggctcat acagctgacg 2461 ggatctccat ccgattccct cgctgcaccc gaatccgaga tgataaggac tggaaatctg 2521 ccactaacct tccccaactc aaggaactgt accagttgtc caaggagaag gcagacttca 2581 ctgtagtggc tggagatgag gggagctcca ctacaggggg tagcagtgaa gagaataagg 2641 gtccctcagg gtctgctgtg tcccgcaagg cccccagcaa gccctcagcc agtaccaaga 2701 aagcagaagg gaagctgagt aactccaaca gcaaagatgg caacatgcag actgcaaagc 2761 cttccgctat gaaggtgggg gagaagctgg ccacaaagTC TTCTCCAGTG AAAGTAGGGG 2821 AGAAGCGGAA AGCTGCTGAT GAGACGCTGT GCCAAACAAA GGTATTGCTG GACATCTTCA 2881 CTGGGGTGCG GCTTTACTTG CCACCCTCCA CACCAGACTT CAGCCGTCTC AGACGCTACT 2941 TTGTGGCATT CGACGGGGAC CTGGTACAGG AATTTGATAT GACTTCAGCC ACGCACGTGC 3001 TGGGTAGCAG GGACAAGAAC CCTGCGGCCC AGCAGGTCTC CCCAGAGTGG ATTTGGGCAT 3061 GTATCCGGAA ACGGAGACTG GTAGCTCCCT GCTACCCAAC TTTCTTGTAC AAAGTGGTTG 3121 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 3181 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAA 3241 CCACACCGCC AAAATCAGCC TTACGCGTTA AGTCgacaat caacctctgg attacaaaat 3301 ttgtgaaaga tt