Construct: ORF TRCN0000470762

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008460.1_s317c1
Derived from:
ccsbBroadEn_00941
DNA Barcode:
AACCACACCGCCAAAATCAGCCTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LIG3 (3980)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470762
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3980 LIG3 DNA ligase 3 NM_013975.4 100% 100%
2 human 3980 LIG3 DNA ligase 3 XM_017024624.1 100% 100%
3 human 3980 LIG3 DNA ligase 3 XM_005257970.4 99.1% 99.1% 1_27del
4 human 3980 LIG3 DNA ligase 3 NM_002311.4 93.7% 92.3% (many diffs)
5 human 3980 LIG3 DNA ligase 3 XM_006721896.3 92.9% 91.5% (many diffs)
6 mouse 16882 Lig3 ligase III, DNA, ATP-dependent NM_001291246.1 87.6% 88.2% (many diffs)
7 mouse 16882 Lig3 ligase III, DNA, ATP-dependent NM_001291247.1 87.6% 88.2% (many diffs)
8 mouse 16882 Lig3 ligase III, DNA, ATP-dependent NM_001291245.1 87.6% 88.1% (many diffs)
9 mouse 16882 Lig3 ligase III, DNA, ATP-dependent NM_010716.3 87.6% 88.1% (many diffs)
10 mouse 16882 Lig3 ligase III, DNA, ATP-dependent XM_006532350.3 87.6% 88.1% (many diffs)
11 mouse 16882 Lig3 ligase III, DNA, ATP-dependent XM_006532348.3 83.2% 83.7% (many diffs)
12 mouse 16882 Lig3 ligase III, DNA, ATP-dependent XM_006532347.3 83.1% 83.6% (many diffs)
13 mouse 16882 Lig3 ligase III, DNA, ATP-dependent XM_006532353.3 78.1% 77% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3093
ORF length:
3027
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc tttggctttc aagatcttct ttccacaaac cctccgtgca ctcagccgaa
121aagaactgtg cctattccga aaacatcact ggcgtgatgt aagacaattc agccagtggt
181cagaaacaga tctgcttcat ggacatcccc tcttcctgag aagaaagcct gttctatcat
241tccagggaag ccatctaaga tcacgtgcca cctaccttgt tttcttgcca gggttgcatg
301tgggactctg cagtggcccc tgtgagatgg ctgagcaacg gttctgtgtg gactatgcca
361agcgtggcac agctggctgc aaaaaatgca aggaaaagat tgtgaagggc gtatgccgaa
421ttggcaaagt ggtgcccaat cccttctcag agtctggggg tgatatgaaa gagtggtacc
481acattaaatg catgtttgag aaactagagc gggcccgggc caccacaaaa aaaatcgagg
541acctcacaga gctggaaggc tgggaagagc tggaagataa tgagaaggaa cagataaccc
601agcacattgc agatctgtct tctaaggcag caggtacacc aaagaagaaa gctgttgtcc
661aggctaagtt gacaaccact ggccaggtga cttctccagt gaaaggcgcc tcatttgtca
721ccagtaccaa tccccggaaa ttttctggct tttcagccaa gcccaacaac tctggggaag
781ccccctcgag ccccacccct aagagaagtc tgtcttcaag caaatgtgac cccaggcata
841aggactgtct gctacgggag tttcgaaagt tatgcgccat ggtggccgat aatcctagct
901acaacacgaa gacccagatc atccaggact tccttcggaa aggctcagca ggagatggtt
961tccacggtga tgtgtaccta acagtgaagc tgctgctgcc aggagtcatt aagactgttt
1021acaacttgaa cgataagcag attgtgaagc ttttcagtcg catttttaac tgcaacccag
1081atgatatggc acgggaccta gagcagggtg acgtgtcaga gacaatcaga gtcttctttg
1141agcagagcaa gtctttcccc ccagctgcca agagcctcct taccatccag gaagtggatg
1201agttccttct gcggctgtcc aagctcacca aggaggatga gcagcaacag gccctacagg
1261acattgcctc caggtgtaca gccaatgacc ttaaatgcat catcaggttg atcaaacatg
1321atctgaagat gaactcaggt gcaaaacatg tgttagacgc ccttgacccc aatgcctatg
1381aagccttcaa agcctcgcgc aacctgcagg atgtggtgga gcgggtcctt cacaacgcgc
1441aggaggtgga gaaggagccg ggccagagac gagctctgag cgtccaggcc tcgctgatga
1501cacctgtgca gcccatgttg gcggaggcct gcaagtccgt tgagtatgca atgaagaaat
1561gtcccaatgg catgttctct gagatcaagt acgatggaga gcgagtccag gtgcataaga
1621atggagacca cttcagctac ttcagccgca gtctcaagcc cgtccttcct cacaaggtgg
1681cccactttaa ggactacatt ccccaggctt ttcctggggg ccacagcatg atcttggatt
1741ctgaagtgct tctgattgac aacaagacag gcaaaccact gccctttggg actctgggag
1801tacacaagaa agcagccttc caggatgcta atgtctgcct gtttgttttt gattgtatct
1861actttaatga tgtcagcttg atggacagac ctctgtgtga gcggcggaag tttcttcatg
1921acaacatggt tgaaattcca aaccggatca tgttctcaga aatgaagcga gtcacaaaag
1981ctttggactt ggctgacatg ataacccggg tgatccagga gggattggag gggctggtgc
2041tgaaggatgt gaagggtaca tatgagcctg ggaagcggca ctggctgaaa gtgaagaaag
2101actatttgaa cgagggggcc atggccgaca cagctgacct ggtggtcctt ggagccttct
2161atgggcaagg gagcaaaggc ggcatgatgt caatcttcct catgggctgc tacgaccctg
2221gcagccagaa gtggtgcaca gtcaccaagt gtgcaggagg ccatgatgat gccacgcttg
2281cccgcctgca gaatgaacta gacatggtga agatcagcaa ggaccccagc aaaataccca
2341gctggttgaa ggtcaacaag atctactatc ctgacttcat cgtcccagac ccaaagaaag
2401ctgccgtgtg ggagatcaca ggggctgaat tctccaaatc ggaggctcat acagctgacg
2461ggatctccat ccgattccct cgctgcaccc gaatccgaga tgataaggac tggaaatctg
2521ccactaacct tccccaactc aaggaactgt accagttgtc caaggagaag gcagacttca
2581ctgtagtggc tggagatgag gggagctcca ctacaggggg tagcagtgaa gagaataagg
2641gtccctcagg gtctgctgtg tcccgcaagg cccccagcaa gccctcagcc agtaccaaga
2701aagcagaagg gaagctgagt aactccaaca gcaaagatgg caacatgcag actgcaaagc
2761cttccgctat gaaggtgggg gagaagctgg ccacaaagTC TTCTCCAGTG AAAGTAGGGG
2821AGAAGCGGAA AGCTGCTGAT GAGACGCTGT GCCAAACAAA GGTATTGCTG GACATCTTCA
2881CTGGGGTGCG GCTTTACTTG CCACCCTCCA CACCAGACTT CAGCCGTCTC AGACGCTACT
2941TTGTGGCATT CGACGGGGAC CTGGTACAGG AATTTGATAT GACTTCAGCC ACGCACGTGC
3001TGGGTAGCAG GGACAAGAAC CCTGCGGCCC AGCAGGTCTC CCCAGAGTGG ATTTGGGCAT
3061GTATCCGGAA ACGGAGACTG GTAGCTCCCT GCTACCCAAC TTTCTTGTAC AAAGTGGTTG
3121ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
3181GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAA
3241CCACACCGCC AAAATCAGCC TTACGCGTTA AGTCgacaat caacctctgg attacaaaat
3301ttgtgaaaga tt

Download FASTA (ORF) (Full)