Construct: ORF TRCN0000470791
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009767.1_s317c1
- Derived from:
- ccsbBroadEn_06273
- DNA Barcode:
- AAGACTGCCTCACCCCCACCCACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GIP (2695)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470791
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2695 | GIP | gastric inhibitory polypeptide | NM_004123.3 | 99.7% | 99.3% | 307A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 528
- ORF length:
- 459
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtggccacg aagacctttg cTCTGCTGCT GCTGTCCCTG TTCCTGGCAG 121 TGGGACTAGG AGAGAAGAAA GAGGGTCACT TCAGCGCTCT CCCCTCCCTG CCTGTTGGAT 181 CTCATGCTAA GGTGAGCAGC CCTCAACCTC GAGGCCCCAG GTACGCGGAA GGGACTTTCA 241 TCAGTGACTA CAGTATTGCC ATGGACAAGA TTCACCAACA AGACTTTGTG AACTGGCTGC 301 TGGCCCAAAA GGGGAAGAAG AATGACTGGA AACACAACAT CACCCAGAGG GAGGCTCGGG 361 CGCTGGAGCT GGCCGGTCAA GCTAATAGGA AGGAGGAGGA GGCAGTGGAG CCACAGAGCT 421 CCCCAGCCAA GAACCCCAGC GATGAAGATT TGCTGCGGGA CTTGCTGATT CAAGAGCTGT 481 TGGCCTGCTT GCTGGATCAG ACAAACCTCT GCAGGCTCAG GTCTCGGTTG CCAACTTTCT 541 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 601 CGTAGTAATG AACTAGCCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 661 GTGGAAAGGA CGAAAGACTG CCTCACCCCC ACCCACAACG CGTTAAGTCg acaatcaacc 721 tctggattac aaaatttgtg aaagatt