Construct: ORF TRCN0000470838

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011122.3_s317c1
Derived from:
ccsbBroadEn_14971
DNA Barcode:
TTACGCTACGGTTAGGACGGTGCT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PMVK (10654)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470838
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10654 PMVK phosphomevalonate kinase NM_006556.4 100% 100%
2 human 10654 PMVK phosphomevalonate kinase NM_001323011.3 84.4% 86.9% (many diffs)
3 human 10654 PMVK phosphomevalonate kinase NM_001323012.3 60.9% 60.9% 0_1ins225
4 human 10654 PMVK phosphomevalonate kinase NM_001348696.2 60.9% 53.6% 0_1ins106;50_51ins119
5 mouse 68603 Pmvk phosphomevalonate kinase NM_026784.3 86.6% 83.3% (many diffs)
6 mouse 68603 Pmvk phosphomevalonate kinase NM_001310640.1 52.4% 52% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
642
ORF length:
576
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc cccgctggga ggcgccccgc ggctggtact gctgttcagc ggcaagagga
121aatccgggaa ggacttcgtg accgaggcgc tgcagagcag acttggagct gatgtctgtg
181ctgtcctccg gctctctggt ccactcaagg aacagtatgc tcaggagcat ggcttgaact
241tccagagact cctggacacc agcacctaca aggaggcctt TCGGAAGGAC ATGATCCGCT
301GGGGAGAGGA GAAACGCCAG GCTGACCCAG GCTTCTTTTG CAGGAAGATT GTGGAGGGCA
361TCTCCCAGCC CATCTGGCTG GTGAGTGACA CACGGAGAGT GTCTGACATC CAGTGGTTTC
421GGGAGGCCTA TGGGGCCGTG ACGCAGACGG TCCGCGTTGT AGCGTTGGAG CAGAGCCGAC
481AGCAGCGGGG CTGGGTGTTC ACGCCAGGGG TGGACGATGC TGAGTCAGAA TGTGGCCTGG
541ACAACTTCGG GGACTTTGAC TGGGTCATCG AGAACCATGG AGTTGAACAG CGCCTGGAGG
601AGCAGTTGGA GAACCTGATA GAATTTATCC GCTCCAGACT TTACCCAACT TTCTTGTACA
661AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
721AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
781AGGACGATTA CGCTACGGTT AGGACGGTGC TACGCGTTAA GTCgacaatc aacctctgga
841ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)

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