Construct: ORF TRCN0000470871
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011278.1_s317c1
- Derived from:
- ccsbBroadEn_00811
- DNA Barcode:
- TGCCTTAAGTTGCGCTGATTCTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ICAM1 (3383)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470871
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3383 | ICAM1 | intercellular adhesion mole... | NM_000201.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1662
- ORF length:
- 1596
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tcccagcagc ccccggcccg cgctgcccgc actcctggtc ctgctcgggg 121 ctctgttccc aggacctggc aatgcccaga catctgtgtc cccctcaaaa gtcatcctgc 181 cccggggagg ctccgtgctg gtgacatgca gcacctcctg tgaccagccc aagttgttgg 241 gcatagagac cccgttgcct aaaaaggagt tgctcctgcc tgggaacaac cggaaggtgt 301 atgaactgag caatgtgcaa gaagatagcc aaccaatgtg ctattcaaac tgccctgatg 361 ggcagtcaac agctaaaacc ttcctcaccg tgtactggac tccagaacgg gtggaactgg 421 cacccctccc ctcttggcag ccagtgggca agaaccttac cctacgctgc caggtggagg 481 gtggggcacc ccgggccaac ctcaccgtgg tgctgctccg tggggagaag gagctgaaac 541 gggagccagc tgtgggggag cccgctgagg tcacgaccac ggtgctggtg aggagagatc 601 accatggagc caatttctcg tgccgcactg aactggacct gcggccccaa gggctggagc 661 tgtttgagaa cacctcggcc ccctaccagc tccagacctt tgtcctgcca gcgactcccc 721 cacaacttgt cagcccccgg gtcctagagg tggacacgca ggggaccgtg gtctgttccc 781 tggacgggct gttcccagtc tcggaggccc aggtccacct ggcactgggg gaccagaggt 841 tgaaccccac agtcacctat ggcaacgact ccttctcggc caaggcctca gtcagtgtga 901 ccgcagagga cgagggcacc cagcggctga cgtgtgcagt aatactgggg aaccagagcc 961 aggagacact gcagacagtg accatctaca gctttccggc gcccaacgtg attctgacga 1021 agccagaggt ctcagaaggg accgaggtga cagtgaagtg tgaggcccac cctagagcca 1081 aggtgacgct gaatggggtt ccagcccagc cactgggccc gagggcccag ctcctgctga 1141 aggccacccc agaggacaac gggcgcagct tctcctgcTC TGCAACCCTG GAGGTGGCCG 1201 GCCAGCTTAT ACACAAGAAC CAGACCCGGG AGCTTCGTGT CCTGTATGGC CCCCGACTGG 1261 ACGAGAGGGA TTGTCCGGGA AACTGGACGT GGCCAGAAAA TTCCCAGCAG ACTCCAATGT 1321 GCCAGGCTTG GGGGAACCCA TTGCCCGAGC TCAAGTGTCT AAAGGATGGC ACTTTCCCAC 1381 TGCCCATCGG GGAATCAGTG ACTGTCACTC GAGATCTTGA GGGCACCTAC CTCTGTCGGG 1441 CCAGGAGCAC TCAAGGGGAG GTCACCCGCA AGGTGACCGT GAATGTGCTC TCCCCCCGGT 1501 ATGAGATTGT CATCATCACT GTGGTAGCAG CCGCAGTCAT AATGGGCACT GCAGGCCTCA 1561 GCACGTACCT CTATAACCGC CAGCGGAAGA TCAAGAAATA CAGACTACAA CAGGCCCAAA 1621 AAGGGACCCC CATGAAACCG AACACACAAG CCACGCCTCC CTGCCCAACT TTCTTGTACA 1681 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1741 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1801 AGGACGATGC CTTAAGTTGC GCTGATTCTG AACGCGTTAA GTCgacaatc aacctctgga 1861 ttacaaaatt tgtgaaagat t