Construct: ORF TRCN0000470875
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003603.1_s317c1
- Derived from:
- ccsbBroadEn_00104
- DNA Barcode:
- TGATACACGGGCTAGACCCTTGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARL2 (402)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470875
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 402 | ARL2 | ADP ribosylation factor lik... | NM_001667.4 | 100% | 100% | |
| 2 | human | 402 | ARL2 | ADP ribosylation factor lik... | NM_001199745.2 | 85.3% | 85.3% | 336_337ins81 |
| 3 | human | 100528018 | ARL2-SNX15 | ARL2-SNX15 readthrough (NMD... | NR_037650.1 | 19.8% | (many diffs) | |
| 4 | mouse | 56327 | Arl2 | ADP-ribosylation factor-like 2 | NM_019722.3 | 90.7% | 96.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 618
- ORF length:
- 552
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gctcctgacc attctgaaga agatgaagca gaaagagcgg gagctgcgac 121 tgctcatgct tggcctggac aatgctggaa agacaaccat cctgaagaag ttcaatgggg 181 aggacatcga caccatcTCC CCAACGCTGG GCTTCAACAT CAAGACCCTG GAGCACCGAG 241 GATTCAAGCT GAACATCTGG GATGTGGGTG GCCAGAAGTC CCTGCGGTCC TACTGGCGGA 301 ACTACTTTGA GAGCACCGAT GGCCTCATCT GGGTAGTGGA CAGCGCAGAC CGCCAGCGCA 361 TGCAGGACTG CCAGCGGGAG CTCCAGAGCC TGCTGGTGGA GGAGCGCCTG GCCGGAGCAA 421 CCCTCCTCAT CTTTGCTAAT AAGCAGGACC TGCCTGGAGC ACTGTCCTCT AACGCCATCC 481 GCGAGGTCCT GGAGCTGGAC TCCATCCGCA GCCACCACTG GTGCATCCAG GGCTGCAGCG 541 CCGTCACCGG GGAGAACCTG CTGCCGGGCA TCGACTGGCT CCTGGATGAC ATTTCCAGCC 601 GCATTTTCAC AGCTGACTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 661 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 721 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATGATACA CGGGCTAGAC 781 CCTTGTAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt