Construct: ORF TRCN0000470888
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009770.1_s317c1
- Derived from:
- ccsbBroadEn_13138
- DNA Barcode:
- TATCCTTATTAACACAGTCGTGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DRAM2 (128338)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470888
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 128338 | DRAM2 | DNA damage regulated autoph... | NM_001349886.1 | 79.2% | 74.5% | (many diffs) |
| 2 | human | 128338 | DRAM2 | DNA damage regulated autoph... | NM_001349887.1 | 79.2% | 74.5% | (many diffs) |
| 3 | human | 128338 | DRAM2 | DNA damage regulated autoph... | NM_001349888.1 | 79.2% | 74.5% | (many diffs) |
| 4 | human | 128338 | DRAM2 | DNA damage regulated autoph... | NM_001349881.1 | 78.1% | 78.1% | 1_174del |
| 5 | human | 128338 | DRAM2 | DNA damage regulated autoph... | NM_001349882.1 | 78.1% | 78.1% | 1_174del |
| 6 | human | 128338 | DRAM2 | DNA damage regulated autoph... | NM_001349884.1 | 78.1% | 78.1% | 1_174del |
| 7 | human | 128338 | DRAM2 | DNA damage regulated autoph... | NM_001349885.1 | 78.1% | 78.1% | 1_174del |
| 8 | human | 128338 | DRAM2 | DNA damage regulated autoph... | NM_178454.5 | 78.1% | 78.1% | 1_174del |
| 9 | human | 128338 | DRAM2 | DNA damage regulated autoph... | XM_011540708.1 | 78.1% | 78.1% | 1_174del |
| 10 | human | 128338 | DRAM2 | DNA damage regulated autoph... | NM_001349889.1 | 65.3% | 65.3% | 0_1ins216 |
| 11 | human | 128338 | DRAM2 | DNA damage regulated autoph... | NM_001349890.1 | 65.3% | 65.3% | 0_1ins216 |
| 12 | human | 128338 | DRAM2 | DNA damage regulated autoph... | NM_001349891.1 | 65.3% | 65.3% | 0_1ins216 |
| 13 | human | 128338 | DRAM2 | DNA damage regulated autoph... | NM_001349892.1 | 65.3% | 65.3% | 0_1ins216 |
| 14 | human | 128338 | DRAM2 | DNA damage regulated autoph... | NM_001349893.1 | 65.3% | 65.3% | 0_1ins216 |
| 15 | human | 128338 | DRAM2 | DNA damage regulated autoph... | NR_146301.1 | 29.6% | 1_431del;757_758insAGTAAGTGCACTTAGC;1040_2032del | |
| 16 | human | 128338 | DRAM2 | DNA damage regulated autoph... | NR_146302.1 | 29.1% | (many diffs) | |
| 17 | human | 128338 | DRAM2 | DNA damage regulated autoph... | NR_146308.1 | 27.4% | 1_597del;923_924insAGTAAGTGCACTTAGC;1206_2198del | |
| 18 | human | 128338 | DRAM2 | DNA damage regulated autoph... | NR_146303.1 | 26.9% | 1_642del;968_969insAGTAAGTGCACTTAGC;1251_2243del | |
| 19 | human | 128338 | DRAM2 | DNA damage regulated autoph... | NR_146305.1 | 25.2% | (many diffs) | |
| 20 | human | 128338 | DRAM2 | DNA damage regulated autoph... | NR_146306.1 | 25% | (many diffs) | |
| 21 | human | 128338 | DRAM2 | DNA damage regulated autoph... | NR_146307.1 | 24.7% | (many diffs) | |
| 22 | human | 128338 | DRAM2 | DNA damage regulated autoph... | NR_146304.1 | 24.5% | (many diffs) | |
| 23 | mouse | 67171 | Dram2 | DNA-damage regulated autoph... | XM_006501928.1 | 88.8% | 85.6% | (many diffs) |
| 24 | mouse | 67171 | Dram2 | DNA-damage regulated autoph... | NM_001286986.1 | 69.5% | 67% | (many diffs) |
| 25 | mouse | 67171 | Dram2 | DNA-damage regulated autoph... | NM_001286987.1 | 69.5% | 67% | (many diffs) |
| 26 | mouse | 67171 | Dram2 | DNA-damage regulated autoph... | NM_026013.3 | 69.5% | 67% | (many diffs) |
| 27 | mouse | 67171 | Dram2 | DNA-damage regulated autoph... | NM_001025582.2 | 41.1% | 40.8% | (many diffs) |
| 28 | mouse | 67171 | Dram2 | DNA-damage regulated autoph... | XM_006501929.3 | 41.1% | 40.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 690
- ORF length:
- 624
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct aaatattgcg gcagttttat gcattgctac catttatgtt cgttataagc 121 aagttcatgc tctgagtcct gaagagaacg ttatcatcaa attaaacaag gctggccttg 181 tacttGGAAT ACTGAGTTGT TTAGGACTTT CTATTGTGGC AAACTTCCAG AAAACAACCC 241 TTTTTGCTGC ACATGTAAGT GGAGCTGTGC TTACCTTTGG TATGGGCTCA TTATATATGT 301 TTGTTCAGAC CATCCTTTCC TACCAAATGC AGCCCAAAAT CCATGGCAAA CAAGTCTTCT 361 GGATCAGACT GTTGTTGGTT ATCTGGTGTG GAGTAAGTGC ACTTAGCATG CTGACTTGCT 421 CATCAGTTTT GCACAGTGGC AATTTTGGGA CTGATTTAGA ACAGAAACTC CATTGGAACC 481 CCGAGGACAA AGGTTATGTG CTTCACATGA TCACTACTGC AGCAGAATGG TCTATGTCAT 541 TTTCCTTCTT TGGTTTTTTC CTGACTTACA TTCGTGATTT TCAGAAAATT TCTTTACGGG 601 TGGAAGCCAA TTTACATGGA TTAACCCTCT ATGACACTGC ACCTTGCCCT ATTAACAATG 661 AACGAACACG GCTACTTTCC AGAGATATTT GCCCAACTTT CTTGTACAAA GTGGTTGATA 721 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 781 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATATCC 841 TTATTAACAC AGTCGTGGGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 901 tgaaagatt