Construct: ORF TRCN0000470897
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012929.1_s317c1
- Derived from:
- ccsbBroadEn_04635
- DNA Barcode:
- GGATAATCACCTCCGTATTCACTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VPS26B (112936)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470897
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 112936 | VPS26B | VPS26, retromer complex com... | NM_052875.5 | 100% | 100% | |
| 2 | human | 112936 | VPS26B | VPS26, retromer complex com... | XM_011542565.3 | 62.4% | 52.8% | (many diffs) |
| 3 | mouse | 69091 | Vps26b | VPS26 retromer complex comp... | NM_178027.4 | 92.4% | 99.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1074
- ORF length:
- 1008
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag cttcttcggc ttcgggcaga gcgtggaggt ggaaatcctt ctgaacgatg 121 cagagagtag gaagcgggcc gagcacaaga cggaggacgg gaagaaggag aaatatttcc 181 tcttctacga cggggagacg gtctccggga aggtgagcct tgccctcaag aaccccaaca 241 agcggctgga gcaccagggc atcaagatcg agttcatcgg gcagatcgaa ctctactacg 301 atcgcgggaa ccaccatgag tttgtgtccc tggtgaagga cctggcccgg cctggagaga 361 tcacccagtc gcaggccttc gactttgagt ttacccacgt ggagaagccg tatgagtcct 421 acacagggca gaatgtgaag ctacgctatt tccttcgtgc taccatcagc cgccgcctca 481 atgatgttgt caaagagatg gacattgtag ttcacacact cagcacatac ccagagctga 541 actcttccat caagatggag gttgggattg aggactgtct gcacattgaa tttgagtaca 601 ataaatccaa ataccacttg aaagatgtca ttgtagggaa gatatacttc ctgctggtga 661 gaatcaaaat caagcacatG GAGATAGACA TCATCAAGCG AGAAACGACG GGTACAGGCC 721 CCAACGTGTA CCATGAGAAT GACACGATAG CCAAGTACGA GATCATGGAC GGGGCACCAG 781 TGCGAGGAGA GTCCATCCCG ATCCGGCTCT TCCTGGCCGG GTATGAGCTC ACGCCCACCA 841 TGCGGGACAT CAACAAGAAG TTCTCTGTGC GCTATTACCT CAACCTGGTG CTGATAGACG 901 AGGAGGAGCG GCGCTACTTC AAGCAGCAGG AAGTGGTGTT GTGGCGGAAG GGTGACATCG 961 TACGGAAGAG CATGTCCCAC CAGGCGGCCA TCGCCTCACA GCGCTTTGAG GGCACCACCT 1021 CCCTGGGTGA GGTGCGGACC CCCAGCCAGC TGTCTGACAA CAACTGCAGG CAGTACCCAA 1081 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1141 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1201 TATCTTGTGG AAAGGACGAG GATAATCACC TCCGTATTCA CTAACGCGTT AAGTCgacaa 1261 tcaacctctg gattacaaaa tttgtgaaag att