Construct: ORF TRCN0000470921

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016537.1_s317c1
Derived from:
ccsbBroadEn_13550
DNA Barcode:
GAGTTAGAATATGATCAACCGTGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RPUSD3 (285367)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470921
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 285367 RPUSD3 RNA pseudouridine synthase D3 NM_173659.4 97.6% 97.4% 1_24del;100G>C
2 human 285367 RPUSD3 RNA pseudouridine synthase D3 NM_001142547.2 93.3% 93.1% 1_24del;100G>C;260_261ins45
3 human 285367 RPUSD3 RNA pseudouridine synthase D3 XM_024453471.1 79.3% 79.8% (many diffs)
4 human 285367 RPUSD3 RNA pseudouridine synthase D3 NM_001351738.1 75.3% 56.4% (many diffs)
5 human 285367 RPUSD3 RNA pseudouridine synthase D3 XM_024453472.1 69% 66.9% (many diffs)
6 human 285367 RPUSD3 RNA pseudouridine synthase D3 NM_001351737.1 66.8% 66% (many diffs)
7 human 285367 RPUSD3 RNA pseudouridine synthase D3 NM_001351736.2 60.4% 59.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1095
ORF length:
1029
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga cggccgccgt gttttgggcc ggttctggag tggctggcgg cggggcctgg
121gtgtccgccc agtgcccgag cacgcaggct ttggcaccga agcccggcat cagaggcaac
181cccgcggctc ctgccaacgg tcggggcccc tcggggacca gcccttcgcg gggctgctgc
241caaaaaacct cagtcgggag gagctggttg atgcgctgcg ggcagccgtg gtggaccgga
301aaggacctct agtgacgttg aacaagccac agggtctacc agtgacagga aaaccaggag
361agctgacgtt gttctcagtg ctgccagagc tgagccagtc cctagggctc agggagcagg
421agcttcaggt tgtccgagca tctgggaaag aaagctctgg gcttgtactc ctctccagct
481gtccccagac agctagtcgc ctccagaagt acttcaccca tgcacggaga gcccaaaggc
541ccacagccac ctactgtgct gtcactgatg ggatcccagc tgcttctgag gggaagatcc
601aggctgccct gaaactggaa cacattgatg gggtcaatct cacagttcca gtgaaggccc
661catcccgaaa ggacatcctg gaaggtgtca agaagactct cagtcacttt cgtgtggtag
721ccacaggctc tggctgtgcc ctggtccagc tgcagccact gacagtgttc tccagtcaac
781tacaggtgca catggtacta cagctctgcc ctgtgcttgg ggaccacatg tactctgccc
841gtgtgggcac tgtcctgggc cagcgatttc tgctgccagc TGAGAACAAC AAGCCCCAAA
901GACAGGTCCT GGATGAAGCC CTCCTCAGAC GCCTCCACCT GACCCCCTCC CAGGCTGCCC
961AGCTGCCCTT GCACCTCCAC CTACATCGGC TCCTTCTCCC AGGCACCAGG GCCAGGGACA
1021CCCCTGTTGA GCTCCTGGCA CCACTGCCCC CTTATTTCTC CAGGACCCTA CAGTGCCTGG
1081GGCTCCGCTT ACAATACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
1141CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
1201TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GAGTTAGAAT ATGATCAACC
1261GTGTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)