Construct: ORF TRCN0000470923
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008559.1_s317c1
- Derived from:
- ccsbBroadEn_02721
- DNA Barcode:
- CCATACTACGCACCCCCGGTGCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPS27 (23107)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470923
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23107 | MRPS27 | mitochondrial ribosomal pro... | NM_015084.3 | 100% | 100% | |
| 2 | human | 23107 | MRPS27 | mitochondrial ribosomal pro... | NM_001286748.2 | 96.7% | 96.7% | 282_323del |
| 3 | human | 23107 | MRPS27 | mitochondrial ribosomal pro... | NM_001286751.2 | 86.4% | 86.4% | 0_1ins168 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1308
- ORF length:
- 1242
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcctccata gtgcggcgcg ggatgctcct ggcgcggcaa gtggttcttc 121 ctcagctctc tcctgcaggt aaaagatacc tgctttcttc agcctatgta gacagccaca 181 aatgggaagc aagagaaaaa gaacattact gtcttgctga tcttgcatct ttaatggata 241 aaacatttga gagaaagttg cctgttagtt ctttaacaat atcacggctt atagacaaca 301 tttcctctcg ggaagagata gatcatgcag agtattacct ttacaagttt cgacacagcc 361 ccaactgctg gtacctgaga aactggacta tccacacctg gattaggcag tgtctaaaat 421 atgatgcaca agacaaagcc ctatataccc ttgtaaataa ggttcaatat ggaatttttc 481 cagataactt tacattcaat ttactgatgg attctttcat aaagaaagaa aattacaaag 541 atgctttatc tgtggttttt gaggtcatga tgcaagaagc ctttgaagtg ccttccaccc 601 aacttctctc cctctatgtt ttatttcatt gcctggcaaa gaagacagac ttcagttggg 661 aagaggagag gaactttggt gcatcccttt tgcttccagg cctaaaacaa aagaactcag 721 tgggtttcag ttcccagttg tatggctatg cacttcttgg gaaggtggag ttgcagcaag 781 ggctacgggc tgtgtaccac aacatgcctc tgatatggaa accaggctac cttgacagag 841 cccttcaagt gatggagaaa gtggctgccT CCCCAGAAGA CATAAAGCTG TGTAGAGAAG 901 CGCTCGATGT GCTGGGTGCA GTGCTGAAGG CTCTGACTTC AGCTGATGGG GCTTCAGAGG 961 AGCAGTCCCA AAATGATGAA GACAACCAGG GGTCAGAAAA ACTGGTGGAG CAGTTAGACA 1021 TCGAGGAAAC AGAGCAGTCC AAGCTTCCTC AATACCTGGA ACGATTTAAG GCCTTACATT 1081 CTAAGCTTCA AGCTCTGGGC AAAATTGAGT CAGAAGGTCT TTTAAGTCTG ACCACCCAGC 1141 TTGTCAAGGA AAAACTCTCC ACCTGTGAAG CAGAGGACAT CGCCACCTAT GAGCAGAATC 1201 TGCAGCAGTG GCATCTAGAC CTTGTACAGT TGATCCAGAG AGAACAGCAA CAGAGGGAGC 1261 AAGCGAAGCA GGAGTACCAG GCTCAGAAAG CAGCAAAGGC ATCTGCCTAC CCAACTTTCT 1321 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1381 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1441 GTGGAAAGGA CGACCATACT ACGCACCCCC GGTGCGCACG CGTTAAGTCg acaatcaacc 1501 tctggattac aaaatttgtg aaagatt