Construct: ORF TRCN0000470937
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003130.1_s317c1
- Derived from:
- ccsbBroadEn_14015
- DNA Barcode:
- GCTGCCGGGCAAATGATCGGATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATG12 (9140)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470937
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9140 | ATG12 | autophagy related 12 | NM_001277783.2 | 99.5% | 98.6% | 211T>G |
| 2 | human | 9140 | ATG12 | autophagy related 12 | NM_004707.4 | 52.6% | 42.1% | 164_300del;348T>G;360_420del |
| 3 | human | 9140 | ATG12 | autophagy related 12 | NR_033363.2 | 5.3% | (many diffs) | |
| 4 | human | 9140 | ATG12 | autophagy related 12 | NR_033362.2 | 5.3% | (many diffs) | |
| 5 | human | 9140 | ATG12 | autophagy related 12 | NR_073603.2 | 5.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 164
- ORF end:
- 386
- ORF length:
- 222
- Sequence:
-
1 CCAGCTTGGC ACTTGATGTA ATTCTCCTTG GAATTTGCCC TTTTTGAGTT TGGATCTTGG 61 TTCATTCTCA AGCCTCAGAC AGTGGTTCAA AGTTTTTTTC TTCCATTTCA GGTGTCGTGA 121 GGCTAGCATC GATTGATCAA CAAGTTTGTA CAAAAAAGTT GGCATGGCGG AGGAGCCGCA 181 GTCTGTGTTG CAGCTTCCTA CTTCAATTGC TGCTGGAGGG GAAGGACTTA CGGATGTCTC 241 CCCAGAAACA ACCACCCCGG AGCCCCCGTC TTCCGCTGCA GTTTCCCCGG GAACAGAGGA 301 ACCTGCTGGC GACACCAAGA AAAAAATTTA TTTATGTGAA TCAGTCCTTT GCTCCTTCCC 361 CAGACCAAGA AGTGGGAACT CTCTATGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG 421 TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA 481 ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG AGCTGCCGGG 541 CAAATGATCG GATTCACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa 601 agatt