Construct: ORF TRCN0000470946
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004559.1_s317c1
- Derived from:
- ccsbBroadEn_04928
- DNA Barcode:
- CTTCCGCCCTGACGGCCGGCTCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAGEB10 (139422)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470946
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 139422 | MAGEB10 | MAGE family member B10 | NM_182506.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1110
- ORF length:
- 1041
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcctcgaggt cagaagagta aactccgtgc cagggaaaaa cgccgtcagg 121 cccgaggagg gctggaagat ttgatagatg ctctggacat tttagaagag gaggaagaat 181 ctcccccctc tgcctctgct tgtttgaagg atgttttcca gagttcactt gatggggcat 241 ccaacaatcc ccatggactt cgggaagccc aatccaccag cacatctgct acagctgctt 301 cacacacaag acatcccgaa ggagtcaacg accaaatgga agaaaggcca atatgcacac 361 aagatctaga agccactgat agctttccca gaggccctgt agatgagaaa gtaattatat 421 tggtgcatta cttgctgtac aagtaccaaa tgaaagagcc cattacaaag gcagatatgc 481 tgagaaatgt aacccaaatg tccaagagcc agttccctgt aatcctgagc agagcttctg 541 agcacctgga gctgatcttt ggtcttgacc tgaaggaagt ggagcctaat aagcacatct 601 atgtccttgt caacaaacta gatctaggct gtgatgcaaa gctgagtgat gaaacaggcg 661 tgcccaagac tggcctgctg atgactgtcc tgggtattat cTTCACAAAT GGCAATTGTG 721 TCGCTGAGGA GGAAGTCTGG AAAGTGTTTA ACACGATGGG GTTATATGAC GGAATTGAGC 781 ACTTCATGTT TGGGGAGCCC AGGAAGCTCC TTACCAAAGA TTTGGTGAAA GAAAATTACC 841 TGGAGTACCA GCAAGTGCCC AACAGTGATC CTCCACGCTA TCAATTCCTA TGGGGCCCAA 901 GAGCCCATGC TGAAACCAGC AAGATGAAAG TTCTTGAGTT TTTGGCCAAG GTAAATGATA 961 CAGCTCCCAG TGAATTCTCA AACTGGTATA CAGAGGCTTT ACAAGATGAG GAAGAGAGAG 1021 CCAGAGCCAG AGTTGCAGCC AAGGCTCGCG TTAGTGCCAC AGCCGGTGCA CGTTCCAAGG 1081 TTAAGTCCAG CAAGTCCTCC CAACTGCAGT TGCCAACTTT CTTGTACAAA GTGGTTGATA 1141 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1201 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACTTCC 1261 GCCCTGACGG CCGGCTCCGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1321 tgaaagatt