Construct: ORF TRCN0000470983
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015528.2_s317c1
- Derived from:
- ccsbBroadEn_14578
- DNA Barcode:
- CCGCTCGAGGCCCAGAGGACATAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDK8 (1024)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470983
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1024 | CDK8 | cyclin dependent kinase 8 | NM_001318368.2 | 100% | 100% | |
| 2 | human | 1024 | CDK8 | cyclin dependent kinase 8 | NM_001260.3 | 99.7% | 99.7% | 1111_1113delAAG |
| 3 | human | 1024 | CDK8 | cyclin dependent kinase 8 | XM_017020317.2 | 72.6% | 72.6% | 0_1ins378;733_735delAAG |
| 4 | human | 1024 | CDK8 | cyclin dependent kinase 8 | XM_017020318.1 | 62.6% | 62.6% | 0_1ins519 |
| 5 | human | 1024 | CDK8 | cyclin dependent kinase 8 | NM_001346501.2 | 62.5% | 62.5% | 0_1ins519;592_594delAAG |
| 6 | human | 1024 | CDK8 | cyclin dependent kinase 8 | XM_011534865.2 | 62.5% | 62.5% | 0_1ins519;592_594delAAG |
| 7 | mouse | 264064 | Cdk8 | cyclin-dependent kinase 8 | NM_001359990.1 | 93.9% | 98.9% | (many diffs) |
| 8 | mouse | 264064 | Cdk8 | cyclin-dependent kinase 8 | NM_153599.4 | 93.8% | 98.7% | (many diffs) |
| 9 | mouse | 264064 | Cdk8 | cyclin-dependent kinase 8 | XM_006504836.4 | 92.7% | 97.6% | (many diffs) |
| 10 | mouse | 264064 | Cdk8 | cyclin-dependent kinase 8 | XM_006504838.4 | 91.2% | 95.9% | (many diffs) |
| 11 | mouse | 264064 | Cdk8 | cyclin-dependent kinase 8 | XM_006504839.4 | 86.6% | 90.9% | (many diffs) |
| 12 | mouse | 264064 | Cdk8 | cyclin-dependent kinase 8 | XM_030254515.1 | 83.6% | 88.5% | (many diffs) |
| 13 | mouse | 264064 | Cdk8 | cyclin-dependent kinase 8 | XM_006504840.4 | 83.5% | 88.3% | (many diffs) |
| 14 | mouse | 264064 | Cdk8 | cyclin-dependent kinase 8 | NM_001359991.1 | 80.4% | 84.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1455
- ORF length:
- 1389
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ctatgacttt aaagtgaagc tgagcagcga gcgggagcgg gtcgaggacc 121 tgtttgaata cgagggctgc aaagttggcc gaggcactta tggtcacgtc tacaaagcca 181 agaggaaaga tgggaaggat gataaagact atgctttaaa acaaatagaa ggaactggga 241 tctctatgtc ggcatgtaga gaaatagcat tacttcgaga gcttaagcat ccaaacgtca 301 tttctcttca aaaggtgttt ctgtctcatg ctgataggaa ggtgtggctt ctgtttgact 361 atgctgaaca tgacctctgg catataatca agtttcacag agcttctaaa gcaaacaaga 421 agccagttca gttacctcgg ggaatggtga agtcactatt atatcagatc ctagatggta 481 ttcactacct gcatgctaac tgggtgttgc acagagattt gaaacctgct aatattttag 541 ttatgggtga aggtcctgag cgaggaagag taaaaattgc tgacatgggc tttgcccgat 601 tatttaattc acctttgaag cctttagcag atttggatcc agtggttgtt acattctggt 661 accgagcccc tgaactactt cttggagcaa ggcattatac caaagctatt gatatttggg 721 ctatagggtg tatatttgca gaactactaa cgtcagaacc aatatttcac tgtcgacaag 781 aggacatcaa aactagtaat ccttatcacc atgaccagct ggacagaata ttcaatgtaa 841 tgggatttcc tgcagataaa gattgggaag atataaaaaa gatgcctgaa cattcaacat 901 taatgaaaga tttcagaaga aatacgtata ccaactgcag ccttatcaag tatatggaaa 961 aacataaagt taaaccagat agtaaagcat tccacttgct tcagaagctg cttaccatgg 1021 acccaataaa gcgaattacc tcagaaCAGG CTATGCAGGA CCCCTATTTC TTAGAAGACC 1081 CACTTCCTAC ATCAGACGTT TTTGCCGGTT GTCAAATCCC TTACCCAAAA CGAGAATTTT 1141 TAACGGAAGA AGAACCTGAT GACAAAGGAG ACAAAAACCA GCAGCAGCAG CAGGGCAATA 1201 ACCACACTAA TGGAACTGGC CACCCAGGGA ATCAAGACAG CAGTCACACA CAGGGACCCC 1261 CGTTGAAGAA AGTGAGAGTT GTTCCTCCTA CCACTACCTC AGGTGGACTT ATCATGACCT 1321 CAGACTATCA GCGTTCCAAT CCACATGCTG CCTATCCCAA CCCTGGACCA AGCACATCAC 1381 AGCCGCAGAG CAGCATGGGA TACTCAGCTA CCTCCCAGCA GCCTCCACAG TACTCACATC 1441 AGACACATCG GTACTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1501 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1561 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CCGCTCGAGG CCCAGAGGAC 1621 ATAGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt