Construct: ORF TRCN0000470992

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013002.1_s317c1
Derived from:
ccsbBroadEn_00487
DNA Barcode:
GTTTTGGCTACAATTCGCGCAAGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EGR1 (1958)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470992
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1958 EGR1 early growth response 1 NM_001964.3 100% 100%
2 mouse 13653 Egr1 early growth response 1 NM_007913.5 85.9% 91.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1695
ORF length:
1629
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc cgcggccaag gccgagatgc agctgatgtc cccgctgcag atctctgacc
121cgttcggatc ctttcctcac tcgcccacca tggacaacta ccctaagctg gaggagatga
181tgctgctgag caacggggct ccccagttcc tcggcgccgc cggggcccca gagggcagcg
241gcagcaacag cagcagcagc agcagcgggg gcggtggagg cggcgggggc ggcagcaaca
301gcagcagcag cagcagcacc ttcaaccctc aggcggacac gggcgagcag ccctacgagc
361acctgaccgc agagtctttt cctgacatct ctctgaacaa cgagaaggtg ctggtggaga
421ccagttaccc cagccaaacc actcgactgc cccccatcac ctatactggc cgcttttccc
481tggagcctgc acccaacagt ggcaacacct tgtggcccga gcccctcttc agcttggtca
541gtggcctagt gagcatgacc aacccaccgg cctcctcgtc ctcagcacca tctccagcgg
601cctcctccgc ctccgcctcc cagagcccac ccctgagctg cgcagtgcca tccaacgaca
661gcagtcccat ttactcagcg gcacccacct tccccacgcc gaacactgac attttccctg
721agccacaaag ccaggccttc ccgggctcgg cagggacagc gctccagtac ccgcctcctg
781cctaccctgc cgccaagggt ggcttccagg ttcccatgat ccccgactac ctgtttccac
841agcagcaggg ggatctgggc ctgggcaccc cagaccagaa gcccttccag ggcctggaga
901gccgcaccca gcagccttcg ctaacccctc tgtctactat taaggccttt gccactcagt
961cgggctccca ggacctgaag gccctcaata ccagctacca gtcccagctc atcaaaccca
1021gccgcatgcg caagtacccc aaccggccca gcaagacgcc cccccacgaa cgcccttacg
1081cttgcccagt ggagtcctgt gatcgccgct tctcccgctc cgacgagctc acccgccaca
1141tccgcatcca cacaggccag aagcccttcc agtgccgcat ctgcatgcgc aacttcagcc
1201gcagcgacca cctcaccacc cacatccgca cccacacagg cgaaaagccc ttcgcctgcg
1261acatctgtgg aagaaagttt gccaggagcg atgaacgcaa gaggcatacc aagatccact
1321tgcggcagaa ggacaagaaa gcagacaaaa gtgttgtggc ctcttcggcc accTCCTCTC
1381TCTCTTCCTA CCCGTCCCCG GTTGCTACCT CTTACCCGTC CCCGGTTACT ACCTCTTATC
1441CATCCCCGGC CACCACCTCA TACCCATCCC CTGTGCCCAC CTCCTTCTCC TCTCCCGGCT
1501CCTCGACCTA CCCATCCCCT GTGCACAGTG GCTTCCCCTC CCCGTCGGTG GCCACCACGT
1561ACTCCTCTGT TCCCCCTGCT TTCCCGGCCC AGGTCAGCAG CTTCCCTTCC TCAGCTGTCA
1621CCAACTCCTT CAGCGCCTCC ACAGGGCTTT CGGACATGAC AGCAACCTTT TCTCCCAGGA
1681CAATTGAAAT TTGCTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
1741CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
1801TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GTTTTGGCTA CAATTCGCGC
1861AAGCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)