Construct: ORF TRCN0000471023
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002490.1_s317c1
- Derived from:
- ccsbBroadEn_11120
- DNA Barcode:
- ATCATTCGCACAACCACGAACTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCL4 (6351)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471023
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6351 | CCL4 | C-C motif chemokine ligand 4 | NM_002984.4 | 99.2% | 98.9% | 237_238delATinsGA |
| 2 | human | 388372 | CCL4L1 | C-C motif chemokine ligand ... | NM_207007.3 | 97.4% | 95.6% | (many diffs) |
| 3 | human | 9560 | CCL4L2 | C-C motif chemokine ligand ... | NM_001291468.2 | 92% | 90.2% | (many diffs) |
| 4 | human | 9560 | CCL4L2 | C-C motif chemokine ligand ... | NM_001291475.2 | 73.4% | 65% | (many diffs) |
| 5 | human | 9560 | CCL4L2 | C-C motif chemokine ligand ... | NM_001291473.2 | 73.2% | 61.3% | (many diffs) |
| 6 | human | 9560 | CCL4L2 | C-C motif chemokine ligand ... | NM_001291472.2 | 72.7% | 62.2% | (many diffs) |
| 7 | human | 9560 | CCL4L2 | C-C motif chemokine ligand ... | NM_001291471.2 | 69% | 58.4% | (many diffs) |
| 8 | human | 9560 | CCL4L2 | C-C motif chemokine ligand ... | NM_001291469.2 | 68.1% | 67.3% | (many diffs) |
| 9 | human | 9560 | CCL4L2 | C-C motif chemokine ligand ... | NM_001291470.2 | 68.1% | 67.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 198
- ORF end:
- 474
- ORF length:
- 276
- Sequence:
-
1 CCACACTGAG TGGGTGGAGA CTGAAGTTAG GCCAGCTTGG CACTTGATGT AATTCTCCTT 61 GGAATTTGCC CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA 121 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGCTAGCAT CGATTGATCA ACAAGTTTGT 181 ACAAAAAAGT TGGCACCATG AAGCTCTGCG TGACTGTCCT GTCTCTCCTC ATGCTAGTAG 241 CTGCCTTCTG CTCTCCAGCG CTCTCAGCAC CAATGGGCTC AGACCCTCCC ACCGCCTGCT 301 GCTTTTCTTA CACCGCGAGG AAGCTTCCTC GCAACTTTGT GGTAGATTAC TATGAGACCA 361 GCAGCCTCTG CTCCCAGCCA GCTGTGGTAT TCCAAACCAA AAGAAGCAAG CAAGTCTGTG 421 CTGATCCCAG TGAGACCTGG GTCCAGGAGT ACGTGTATGA CCTGGAACTG AACTTGCCAA 481 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 541 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 601 TATCTTGTGG AAAGGACGAA TCATTCGCAC AACCACGAAC TCAACGCGTT AAGTCgacaa 661 tcaacctctg gattacaaaa tttgtgaaag att