Construct: ORF TRCN0000471071
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008915.1_s317c1
- Derived from:
- ccsbBroadEn_00918
- DNA Barcode:
- TGATATTCAATCGGTACTACCACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KRT6B (3854)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471071
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3854 | KRT6B | keratin 6B | NM_005555.4 | 100% | 100% | |
| 2 | human | 286887 | KRT6C | keratin 6C | NM_173086.5 | 98.5% | 98.7% | (many diffs) |
| 3 | human | 3853 | KRT6A | keratin 6A | NM_005554.4 | 97.5% | 98.5% | (many diffs) |
| 4 | mouse | 16687 | Krt6a | keratin 6A | NM_008476.3 | 85.4% | 85.8% | (many diffs) |
| 5 | mouse | 16688 | Krt6b | keratin 6B | NM_010669.2 | 85% | 84.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1758
- ORF length:
- 1692
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaacg 61 ttggcatggc cagcacatcc accaccatca ggagccacag cagcagccgc cggggtttca 121 gtgccaactc agccaggctc cctggggtca gccgctctgg cttcagcagc atctccgtgt 181 cccgctccag gggcagtggt ggcctgggtg gcgcatgtgg aggagctggc tttggcagcc 241 gcagtctgta tggcctgggg ggctccaaga ggatctccat tggagggggc agctgtgcca 301 tcagtggcgg ctatggcagc agagccggag gcagctatgg ctttggtggc gccgggagtg 361 gatttggttt cggtggtgga gccggcattg gctttggtct gggtggtgga gccggccttg 421 ctggtggctt tgggggccct ggcttccctg tgtgcccccc tggaggcatc caagaggtca 481 ctgtcaacca gagtctcctg actcccctca acctgcaaat tgaccccgcc atccagcggg 541 tgcgggccga ggagcgtgag cagatcaaga ccctcaacaa caagtttgcc tccttcatcg 601 acaaggtgcg gttcctagag cagcagaaca aggttctgga caccaagtgg accctgctgc 661 aggagcaggg caccaagact gtgaggcaga acctggagcc gttgttcgag cagtacatca 721 acaacctcag gaggcagctg gacaacatcg tgggggaacg gggtcgtctg gactcggagc 781 tgagaaacat gcaggacctg gtggaggacc tcaagaacaa atatgaggat gaaatcaaca 841 agcgcacagc agcagagaat gaatttgtga ctctgaagaa ggatgtggat gctgcctaca 901 tgaacaaggt tgaactgcaa gccaaggcag acactcttac agatgagatc aacttcctga 961 gagccttgta tgatgcagag ctgtcccaga tgcagaccca catctcagac acatccgtgg 1021 tgctatccat ggacaacaac cgcaacctgg acctggacag catcatcgct gaggtcaagg 1081 cccaatatga ggagattgct cagaggagca gggctgaggc tgagtcctgg taccagacaa 1141 agtacgagga gctgcagatc acagcaggca gacatgggga cgacctgcgc aacaccaagc 1201 aggagattgc tgagatcaac cgcatgatcc agaggctgag atctgagatc gaccacgtca 1261 agaagcagtg tgccaaccTA CAGGCCGCCA TTGCTGATGC TGAGCAGCGT GGGGAGATGG 1321 CCCTCAAGGA TGCTAAGAAC AAGCTGGAAG GGCTGGAGGA TGCCCTGCAG AAGGCCAAGC 1381 AGGACCTGGC CCGGCTGCTG AAGGAGTACC AGGAGCTGAT GAACGTCAAG CTGGCCCTGG 1441 ATGTGGAGAT CGCCACCTAC CGCAAGCTGC TGGAGGGCGA GGAGTGCAGG CTGAATGGCG 1501 AAGGCGTTGG ACAAGTCAAC ATCTCTGTAG TGCAGTCCAC CGTCTCCAGT GGCTATGGCG 1561 GTGCCAGCGG TGTCGGCAGT GGCTTAGGCC TGGGTGGAGG AAGCAGCTAC TCCTATGGCA 1621 GTGGTCTTGG CGTTGGAGGC GGCTTTAGTT CCAGCAGCGG CAGAGCCACT GGGGGTGGCC 1681 TCAGCTCTGT TGGAGGCGGC AGTTCCACCA TCAAGTACAC CACCACCTCC TCCTCCAGCA 1741 GGAAGAGCTA CAAGCACTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1801 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1861 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATGATATT CAATCGGTAC 1921 TACCACAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt