Construct: ORF TRCN0000471071

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008915.1_s317c1
Derived from:
ccsbBroadEn_00918
DNA Barcode:
TGATATTCAATCGGTACTACCACA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KRT6B (3854)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471071
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3854 KRT6B keratin 6B NM_005555.4 100% 100%
2 human 286887 KRT6C keratin 6C NM_173086.5 98.5% 98.7% (many diffs)
3 human 3853 KRT6A keratin 6A NM_005554.4 97.5% 98.5% (many diffs)
4 mouse 16687 Krt6a keratin 6A NM_008476.3 85.4% 85.8% (many diffs)
5 mouse 16688 Krt6b keratin 6B NM_010669.2 85% 84.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1758
ORF length:
1692
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaacg
61ttggcatggc cagcacatcc accaccatca ggagccacag cagcagccgc cggggtttca
121gtgccaactc agccaggctc cctggggtca gccgctctgg cttcagcagc atctccgtgt
181cccgctccag gggcagtggt ggcctgggtg gcgcatgtgg aggagctggc tttggcagcc
241gcagtctgta tggcctgggg ggctccaaga ggatctccat tggagggggc agctgtgcca
301tcagtggcgg ctatggcagc agagccggag gcagctatgg ctttggtggc gccgggagtg
361gatttggttt cggtggtgga gccggcattg gctttggtct gggtggtgga gccggccttg
421ctggtggctt tgggggccct ggcttccctg tgtgcccccc tggaggcatc caagaggtca
481ctgtcaacca gagtctcctg actcccctca acctgcaaat tgaccccgcc atccagcggg
541tgcgggccga ggagcgtgag cagatcaaga ccctcaacaa caagtttgcc tccttcatcg
601acaaggtgcg gttcctagag cagcagaaca aggttctgga caccaagtgg accctgctgc
661aggagcaggg caccaagact gtgaggcaga acctggagcc gttgttcgag cagtacatca
721acaacctcag gaggcagctg gacaacatcg tgggggaacg gggtcgtctg gactcggagc
781tgagaaacat gcaggacctg gtggaggacc tcaagaacaa atatgaggat gaaatcaaca
841agcgcacagc agcagagaat gaatttgtga ctctgaagaa ggatgtggat gctgcctaca
901tgaacaaggt tgaactgcaa gccaaggcag acactcttac agatgagatc aacttcctga
961gagccttgta tgatgcagag ctgtcccaga tgcagaccca catctcagac acatccgtgg
1021tgctatccat ggacaacaac cgcaacctgg acctggacag catcatcgct gaggtcaagg
1081cccaatatga ggagattgct cagaggagca gggctgaggc tgagtcctgg taccagacaa
1141agtacgagga gctgcagatc acagcaggca gacatgggga cgacctgcgc aacaccaagc
1201aggagattgc tgagatcaac cgcatgatcc agaggctgag atctgagatc gaccacgtca
1261agaagcagtg tgccaaccTA CAGGCCGCCA TTGCTGATGC TGAGCAGCGT GGGGAGATGG
1321CCCTCAAGGA TGCTAAGAAC AAGCTGGAAG GGCTGGAGGA TGCCCTGCAG AAGGCCAAGC
1381AGGACCTGGC CCGGCTGCTG AAGGAGTACC AGGAGCTGAT GAACGTCAAG CTGGCCCTGG
1441ATGTGGAGAT CGCCACCTAC CGCAAGCTGC TGGAGGGCGA GGAGTGCAGG CTGAATGGCG
1501AAGGCGTTGG ACAAGTCAAC ATCTCTGTAG TGCAGTCCAC CGTCTCCAGT GGCTATGGCG
1561GTGCCAGCGG TGTCGGCAGT GGCTTAGGCC TGGGTGGAGG AAGCAGCTAC TCCTATGGCA
1621GTGGTCTTGG CGTTGGAGGC GGCTTTAGTT CCAGCAGCGG CAGAGCCACT GGGGGTGGCC
1681TCAGCTCTGT TGGAGGCGGC AGTTCCACCA TCAAGTACAC CACCACCTCC TCCTCCAGCA
1741GGAAGAGCTA CAAGCACTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
1801TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
1861GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATGATATT CAATCGGTAC
1921TACCACAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)