Construct: ORF TRCN0000471135
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015048.1_s317c1
- Derived from:
- ccsbBroadEn_00731
- DNA Barcode:
- TACAGGGCTCTCTACTACTAATAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HBZ (3050)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471135
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3050 | HBZ | hemoglobin subunit zeta | NM_005332.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 492
- ORF length:
- 426
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tctgaccaag actgagagga ccatcattgt gtccatgtgg gccaagatct 121 ccacgcaggc cgacaccatc ggcaccgaga ctctggagag gctcttccTC AGCCACCCGC 181 AGACCAAGAC CTACTTCCCG CACTTCGACC TGCACCCGGG GTCCGCGCAG TTGCGCGCGC 241 ACGGCTCCAA GGTGGTGGCC GCCGTGGGCG ACGCGGTGAA GAGCATCGAC GACATCGGCG 301 GCGCCCTGTC CAAGCTGAGC GAGCTGCACG CCTACATCCT GCGCGTGGAC CCGGTCAACT 361 TCAAGCTCCT GTCCCACTGC CTGCTGGTCA CCCTGGCCGC GCGCTTCCCC GCCGACTTCA 421 CGGCCGAGGC CCACGCCGCC TGGGACAAGT TCCTATCGGT CGTATCCTCT GTCCTGACCG 481 AGAAGTACCG CTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 541 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 601 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATAC AGGGCTCTCT ACTACTAATA 661 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t