Construct: ORF TRCN0000471157
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000984.1_s317c1
- Derived from:
- ccsbBroadEn_12766
- DNA Barcode:
- GGAGTAGAAGCTGCTTGCCGATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OBSCN (84033)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471157
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84033 | OBSCN | obscurin, cytoskeletal calm... | NM_001271223.2 | 1.6% | 1.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 567
- ORF length:
- 498
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcactgcac agtggatgga gaaactgtga ttcttgtgct gggaggtggt 121 gggGCTTCTC CCAGCTACAT GGCCAGTGCC AAGCTTGGGA AGGTTCTGTT TCTCCTACTT 181 TCTCGTCTAG GCCCTCCTAC CCTAGGGTGG TCTTCCTGAC CTGGGCAGTA TCTTTGACCT 241 CGTGTGTCCC TCCTTGTCCA TCCCCAGAGC CCAAGGTGGT GTTTGCCAAG GAGCAGCCAG 301 CACACAGGGA GGTGCAGGCT GAGGCGGGGG CCAGTGCCAC GCTGAGCTGC GAGGTGGCCC 361 AGGCCCAGAC AGAGGTGACG TGGTACAAGG ATGGGAAGAA GCTGAGTTCC AGCTCGAAAG 421 TGCGCGTGGA GGCCGTGGGC TGCACACGGA GGCTGGTGGT GCAGCAGGCG GGCCAGGCAG 481 AGGCCGGGGA GTACAGCTGC GAGGCAGGGG GTCAGCAGCT CTCCTTCCGC CTGCAGGTGG 541 CAGGTCAGTG CTTTGGGGAT GCTGAGTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 601 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 661 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGGAGTAGA 721 AGCTGCTTGC CGATTCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 781 aagatt