Construct: ORF TRCN0000471198
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010980.1_s317c1
- Derived from:
- ccsbBroadEn_15385
- DNA Barcode:
- TTAAGGCGGCTCCGCGGCTTCGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLK3 (1198)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471198
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1198 | CLK3 | CDC like kinase 3 | NM_003992.4 | 95.3% | 95.3% | 470_538del |
| 2 | human | 1198 | CLK3 | CDC like kinase 3 | NM_001130028.1 | 73.1% | 73.1% | 1_444del;914_982del |
| 3 | mouse | 102414 | Clk3 | CDC-like kinase 3 | XM_006510739.3 | 88% | 94% | (many diffs) |
| 4 | mouse | 102414 | Clk3 | CDC-like kinase 3 | XM_017313060.1 | 88% | 94% | (many diffs) |
| 5 | mouse | 102414 | Clk3 | CDC-like kinase 3 | NM_007713.4 | 67.6% | 72.2% | (many diffs) |
| 6 | mouse | 102414 | Clk3 | CDC-like kinase 3 | XM_017313061.1 | 54.2% | 57.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1467
- ORF length:
- 1401
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca tcactgtaag cgataccgct cccctgaacc agacccgtac ctgagctacc 121 gatggaagag gaggaggtcc tacagtcggg aacatgaagg gagactgcga tacccgtccc 181 gaagggagcc tcccccacga agatctcggt ccagaagcca tgaccgcctg ccctaccaga 241 ggaggtaccg ggagcgccgt gacagcgata cataccggtg tgaagagcgg agcccatcct 301 ttggagagga ctactatgga ccttcacgtt ctcgtcatcg tcggcgatcg cgggagaggg 361 ggccataccg gacccgcaag catgcccacc actgccacaa acgccgcacc aggtcttgta 421 gcagcgcctc ctcgagaagc caacagagca gtaagcgcag cagccggagt gtggaagatg 481 acaaggaggg tcacctggtg tgccggatcg gcgattggct ccaagagcga tatgagtctc 541 aggttgccct gaagatcatc cgcaacgtgg gcaagtaccg ggaggctgcc cggctagaaa 601 tcaacgtgct caaaaaaatc aaggagaagg acaaagaaaa caagttcctg tgtgtcttga 661 tgtctgactg gttcaacttc cacggtcaca tgtgcatcgc ctttgagctc ctgggcaaga 721 acacctttga gttcctgaag gagaataact tccagcctta ccccctacca catgtccggc 781 acatggccta ccagctctgc cacgccctta gatttctgca tgagaatcag ctgacccata 841 cagacttgaa accagagaac atcctgtttg tgaattctga gtttgaaacc ctctacaatg 901 agcacaagag ctgtgaggag aagtcagtga agaacaccag catccgagtg gctgactttg 961 gcagtgccac atttgaccat gagcaccaca ccaccattgt ggccacccgt cactatcgcc 1021 cgcctgaggt gatccttgag ctgggctggg cacagccctg tgacgtctgg agcattggct 1081 gcattctctt tgagtactac cggggcttca cactcttcca gacccacgaa aaccgagagc 1141 acctggtgat gatggagaag atcctagggc ccatcccatc acacatgatc caccgtacca 1201 ggaagcagaa atatttctac aaagggggcc TAGTTTGGGA TGAGAACAGC TCTGACGGCC 1261 GGTATGTGAA GGAGAACTGC AAACCTCTGA AGAGTTACAT GCTCCAAGAC TCCCTGGAGC 1321 ACGTGCAGCT GTTTGACCTG ATGAGGAGGA TGTTAGAATT TGACCCTGCC CAGCGCATCA 1381 CACTGGCCGA GGCCCTGCTG CACCCCTTCT TTGCTGGCCT GACCCCTGAG GAGCGGTCCT 1441 TCCACACCAG CCGCAACCCA AGCAGATGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1501 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1561 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATTAAGGCG 1621 GCTCCGCGGC TTCGAAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1681 aagatt