Construct: ORF TRCN0000471268
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014909.1_s317c1
- Derived from:
- ccsbBroadEn_07744
- DNA Barcode:
- TCTCTCCAACCGGAGTTAAAAGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KERA (11081)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471268
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11081 | KERA | keratocan | NM_007035.3 | 99.9% | 100% | 69G>A |
| 2 | mouse | 16545 | Kera | keratocan | NM_008438.3 | 82.8% | 85.8% | (many diffs) |
| 3 | mouse | 16545 | Kera | keratocan | XM_006513273.3 | 82.8% | 85.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1122
- ORF length:
- 1056
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc aggcacaatc tgtttcatca tgtgggtgtt attcataaca gacactgtgt 121 ggtctagaag tgtaaggcag gtctatgaag tacatgattc agatgattgg actattcatg 181 acttcgagtg tcccatggaa tgtttctgcc cacccagttt tcctactgct ttatattgtg 241 aaaatagagg tctcaaagaa attcctgcta ttccttcaag aatttggtat ctttatcttc 301 aaaacaacct gatagaaacc attcctgaaa agccatttga gaatgccacc cagctaagat 361 ggataaatct aaacaagaac aaaataacca actacggaat tgaaaaagga gccctaagcc 421 agctgaagaa gttgctcttc ttatttctgg aagataatga gctagaggag gtaccttctc 481 cattgccaag aagtttagaa caattacaat tagctagaaa taaggtgtcc agaattcctc 541 aagggacctt tagcaatctg gagaacctga cccttcttga cctacagaac aacaaattag 601 tggacaatgc ctttcaaaga gacactttta aaggactcaa gaatctcatg cagctaaaca 661 tggccaagaa tgccctgagg aatatgcctc caagattacc agccaataca atgcagttgt 721 ttttagacaa caattccatt GAAGGAATAC CAGAAAATTA TTTTAATGTG ATTCCTAAAG 781 TGGCCTTTTT GAGACTAAAT CACAACAAAC TGTCAGATGA GGGTCTCCCA TCAAGAGGAT 841 TTGATGTATC ATCAATTCTA GATCTTCAAC TGTCGCACAA TCAACTCACA AAGGTTCCCC 901 GAATCAGTGC TCATCTGCAG CACCTTCACC TTGATCATAA CAAAATTAAA AGTGTGAATG 961 TCTCTGTAAT ATGTCCCAGC CCATCCATGC TGCCTGCAGA ACGAGATTCC TTCAGTTATG 1021 GACCTCATCT TCGCTACCTC CGTCTGGATG GAAATGAAAT CAAACCACCA ATTCCAATGG 1081 CTTTAATGAC CTGCTTCAGA CTTCTGCAGG CTGTCATTAT TTACCCAACT TTCTTGTACA 1141 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1201 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1261 AGGACGATCT CTCCAACCGG AGTTAAAAGT CACGCGTTAA GTCgacaatc aacctctgga 1321 ttacaaaatt tgtgaaagat t