Construct: ORF TRCN0000471270
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000318.1_s317c1
- Derived from:
- ccsbBroadEn_09178
- DNA Barcode:
- TTAAGGGTAACATGGCTACGCACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UTP23 (84294)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471270
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84294 | UTP23 | UTP23 small subunit process... | NM_032334.3 | 99.8% | 99.5% | 674A>G |
| 2 | mouse | 78581 | Utp23 | UTP23 small subunit process... | NM_030132.5 | 83.1% | 86.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 813
- ORF length:
- 747
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gatcacaagg cagaaacatg ccaagaagca tcttggcttc ttccgcaaca 121 acttcggagt ccgcgagccg taccagatcc tgctggacgg caccttctgt caggcggcgc 181 tgcggggccg catccagctg cgggagcagc tgccccgcta cctcatgggg gagacgcagc 241 tgtgcaccac aagatgtgtg ttaaaagagc tagaaacatt gggaaaggac ttatatgggg 301 caaaactgat tgcacaaaaa tgccaagttc gaaattgtcc tcatttcaag aatgcagtga 361 gtggatcaga atgtctgctt tccatggttG AAGAGGGAAA TCCTCATCAT TATTTTGTGG 421 CAACACAGGA TCAGAATTTG TCTGTGAAAG TAAAAAAGAA GCCTGGAGTT CCTCTCATGT 481 TTATTATTCA GAACACTATG GTTTTGGACA AACCTTCTCC CAAAACAATT GCCTTTGTAA 541 AAGCAGTGGA GTCAGGTCAG CTTGTCTCAG TGCATGAGAA AGAAAGTATC AAACATCTCA 601 AAGAGGAACA GGGTTTAGTG AAAAACACTG AACAGAGTAG AAGAAAAAAG CGCAAGAAAA 661 TAAGTGGTCC CAATCCTCTT AGTTGTTTGA AGAAAAAGAA AAAGGCACCG GACACACAAT 721 CATCTGCTTC TGAAAAGAGA AGAAAAAGAA AAAGAATTCG GAACAGATCT AACCCAAAAG 781 TACTTTCTGA GAAGCAGAAT GCAGAAGGAG AATGCCCAAC TTTCTTGTAC AAAGTGGTTG 841 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 901 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATT 961 AAGGGTAACA TGGCTACGCA CTACGCGTTA AGTCgacaat caacctctgg attacaaaat 1021 ttgtgaaaga tt