Construct: ORF TRCN0000471321
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010184.1_s317c1
- Derived from:
- ccsbBroadEn_14384
- DNA Barcode:
- TCTGATGCAACCGATTGTGCTTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TCP10L (140290)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471321
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 110091775 | C21orf59-TCP10L | C21orf59-TCP10L readthrough | NR_146638.2 | 5.1% | (many diffs) | |
| 2 | human | 110091775 | C21orf59-TCP10L | C21orf59-TCP10L readthrough | NR_146639.2 | 5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 444
- ORF length:
- 378
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gcgccctctc atctggcacc ttcctggcct ctttccCAGG CCCCAGTTCT 121 GTCCATGCAG CTGTGGGTGC TTCCTGCATT GCGGGTCTCA CGGGGAGGAG ACGAGAGTGC 181 CCCTGGTTGA GTCAGGAAAG AATTCTATCT TCACGTCGCT GCCAGCAAAT GACCACAGCA 241 GCTTCACGAC CTCTGCAGGA ACCTATCTTG GTAAAGAAAC GGGGCCTATG TGGTGGCCGA 301 GCCTCAGGTG TGGCCGAGCT TCAGGTGTGG CCCTTATGCA CAGCACAGCC CAAGCCTGTG 361 GGCACCACTC GCCCTGGGCT GCCTGGCACC TGGACTCCTT CCCATCCTTG GCCGAGGTCT 421 GCGTGGCCCT TCAGGGTCGA CTCTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 481 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 541 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT CTGATGCAAC 601 CGATTGTGCT TGTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 661 att