Construct: ORF TRCN0000471346
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013569.1_s317c1
- Derived from:
- ccsbBroadEn_12953
- DNA Barcode:
- CATCCCCTCTTTAGATTAACTATC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- AP5B1 (91056)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471346
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91056 | AP5B1 | adaptor related protein com... | NM_138368.5 | 25.3% | 10.1% | 1_1965del;2187_2188insC |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 447
- ORF length:
- 381
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gcaggaggca ccggccctgg tacggctgag cctggggtcc catcgggtca 121 agggcccact cccagtgttg aagctccagc cggaggcgct ggagcccatc tactctctgg 181 agctgcgctt ccgtgtggaa ggacagctgt atgcacccct ggaggctgtc catgtgccct 241 gcctgtgtcc tggccgccct gcccgccctc TGCTCCTGCC TCTGCAGCCC CCGATGCCCG 301 GCCCCCGCAC GGCTGGATGT CCATGCCCTT TACACCACAT CCACTGGTCT CACGTGCCAT 361 GCCCACTTGC CACCCCTGTT CGTGAACTTT GCCGACCTCT TTCTGCCTTT CCCGCAGCCT 421 CCAGAGGGGG CCGGGCTGGG CTTCTTTGAG GAGCTCTGGG ATTCCTGCCT GCCAGAGGGT 481 GCTGAGAGTC GTGTGTGGTG TCCACTTGGG CCACAGGGCC TGGAGGGCTT GGTGTCCCGC 541 CACCTGGAGC CTTTTGTGGT GGTGGCCCAG CCTCCTACCA GCTACTGTGT AGCAATCCAC 601 CTGCCCCCGG ACTCAAAGCT GCTGCTGCGG CTGGAGGCGG CCCTGGCAGA TGGAGTGCCT 661 GTGGCCCTGC GGACCGATGA CTGGGCCGTG CTGCCCCTGG CGGGGGACTA CCTCCGTGGG 721 CTGGCGGCTG CTGTCTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC 781 CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT 841 ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG ACATCCCCTC TTTAGATTAA 901 CTATCACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt