Construct: ORF TRCN0000471383
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005157.1_s317c1
- Derived from:
- ccsbBroadEn_15948
- DNA Barcode:
- GCATTGAAAGTTTCGTCTGCGACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SERINC1 (57515)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471383
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57515 | SERINC1 | serine incorporator 1 | NM_020755.4 | 100% | 100% | |
| 2 | mouse | 56442 | Serinc1 | serine incorporator 1 | NM_019760.3 | 89.8% | 95.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1425
- ORF length:
- 1359
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gagcgtcctg gggctgtgct ccatggcgag ctggatacca tgtttgtgtg 121 gaagtgcccc gtgtttgcta tgccgatgct gtcctagtgg aaacaactcc actgtaacta 181 gattgatcta tgcacttttc ttgcttgttg gagtatgtgt agcttgtgta atgttgatac 241 caggaatgga agaacaactg aataagattc ctggattttg tgagaatgag aaaggtgttg 301 tcccttgtaa cattttggtt ggctataaag ctgtatatcg tttgtgcttt ggtttggcta 361 tgttctatct tcttctctct ttactaatga tcaaagtgaa gagtagcagt gatcctagag 421 ctgcagtgca caatggattt tggttcttta aatttgctgc agcaattgca attattattg 481 gggcattctt cattccagaa ggaactttta caactgtgtg gttttatgta ggcatggcag 541 gtgccttttg tttcatcctc atacaactag tcttacttat tgattttgca cattcatgga 601 atgaatcgtg ggttgaaaaa atggaagaag ggaactcgag atgttggtat gcagccttgt 661 tatcagctac agctctgaat tatctgctgt ctttagttgc tatcgtcctg ttctttgtct 721 actacactca tccagccagt tgttcagaaa acaaggcgtt catcagtgtc aacatgctcc 781 tctgcgttgg tgcttctgta atgtctatac tgccaaaaat ccaagaatca caaccaagat 841 ctggtttgtt acagtcttca gtaattacag tctacacaat gtatttgaca tggtcagcta 901 tgaccaatga aCCAGAAACA AATTGCAACC CAAGTCTACT AAGCATAATT GGCTACAATA 961 CAACAAGCAC TGTCCCAAAG GAAGGGCAGT CAGTCCAGTG GTGGCATGCT CAAGGAATTA 1021 TAGGACTAAT TCTCTTTTTG TTGTGTGTAT TTTATTCCAG CATCCGTACT TCAAACAATA 1081 GTCAGGTTAA TAAACTGACT CTAACAAGTG ATGAATCTAC ATTAATAGAA GATGGTGGAG 1141 CTAGAAGTGA TGGATCACTG GAGGATGGGG ACGATGTTCA CCGAGCTGTA GATAATGAAA 1201 GGGATGGTGT CACTTACAGT TATTCCTTCT TTCACTTCAT GCTTTTCCTG GCTTCACTTT 1261 ATATCATGAT GACCCTTACC AACTGGTACA GGTATGAACC CTCTCGTGAG ATGAAAAGTC 1321 AGTGGACAGC TGTCTGGGTG AAAATCTCTT CCAGTTGGAT TGGCATCGTG CTGTATGTTT 1381 GGACACTCGT GGCACCACTT GTTCTTACAA ATCGTGATTT TGACTGCCCA ACTTTCTTGT 1441 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1501 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1561 GAAAGGACGA GCATTGAAAG TTTCGTCTGC GACAACGCGT TAAGTCgaca atcaacctct 1621 ggattacaaa atttgtgaaa gatt