Construct: ORF TRCN0000471398
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000291.1_s317c1
- Derived from:
- ccsbBroadEn_13604
- DNA Barcode:
- TTCAAAGAACAGGAACGGGCTCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FMN1 (342184)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471398
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 342184 | FMN1 | formin 1 | NM_001103184.4 | 41.8% | 41.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1578
- ORF length:
- 1509
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagaacgtt gataattctc tggatggcag tgatgtgtca gaaccagcaa 121 aaccagaagc cggacttgaa gtggctcagt cgatcctgag taagttctct atgaaatccc 181 tatttgggtt tacgagtaaa ttagaatctg tgaatccaga agaggaagat gctgtgctga 241 aagcattcca tagtttagac gtgaatccca catctcagca ggatgattcc agcaatggct 301 tggatccaca ggaagcaggg tctcgggttt cacctgacct cggaaatgat gagaaaattg 361 caagcgtgga gacagaatca gagggaagtc agagaaaaga agcagggaca tctctccttg 421 ctcaagagct gcttccactg tctactttga aagggacaaa agacgatgtc atttgtgttc 481 gtgggaccct ggtgcacacc accagtgatt ctgactctga tgatggtggc caggagccag 541 aggagggaag cagcaccaat ggcccaaagt cccctagtgt tgttttatct gagccatctc 601 aggagtccaa agaaaaccca ggaggtttca gggaaaatac tgtcactggg gaaatgaatg 661 gtgcagagct ctgtgcagaa gatcctcaaa ggattccacc tgagatgagc agcaaactgg 721 aagctggcaa cggtggtctt cagacagagc gtcgccccag ccaggaccag gtgggagaag 781 aagggtccca agacctacct gcagtaacaa accagaattc gagtgttggt atcactgaga 841 gtgcctcttc taaaaaagaa gtctctggag agaagtcatt ccaacttcca gcttttttca 901 gtgggcttcg tgtgctgaag aagggggcta ccgctgaggg aggagagacc atcacggaaa 961 ttaaaccaaa ggatggggac ttagctttgc tcaaattgac gcagcctgtg cagaagtcct 1021 tagtgcaggc agggctgcag acagtgaaga gtgagaagaa agcaactgat ccgaaggcca 1081 ctcccactct cctggagcag ctctctctgc tgctcaacat tgacatgccc aaaactgaac 1141 cgaagggagc agaccctgag TCGCCCAGGA GAGAAGAGAT GGGCTGCAAT GCTGACCAAG 1201 AGAGCCAGAG CGGCCCTGGA GTACCACAAA CCCAGGGTGG TGAGGTCAAG CCAAAGTCGC 1261 CAGAGACAGC CCTGGAGGCT TTTAAAGCCC TATTCATCCG GCCCCCCAGA AAGGGGACCA 1321 CAGCTGACAC CTCTGAGCTG GAAGCTCTCA AGCGCAAGAT GAGACATGAG AAGGAGTCGC 1381 TAAGAGCTGT GTTTGAACGG TCCAATTCTA AGCCAGCGGA TGGCCCCTCT GATTCCAAAA 1441 GTCCTGACCA CAGCCTGACT GAGCAGGATG ACAGGACTCC TGGCAGACTT CAAGCTGTCT 1501 GGCCACCCCC AAAGACAAAA GACACAGAAG AAAAAGTGGG ACTGAAGTAC ACTGAAGCAG 1561 GTAATGTGAA AGGAAGCTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1621 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1681 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATTCAAAG AACAGGAACG 1741 GGCTCATACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt