Construct: ORF TRCN0000471423
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011904.1_s317c1
- Derived from:
- ccsbBroadEn_10232
- DNA Barcode:
- ACCATCTTTTCTAAATGGATCATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDIA5 (10954)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471423
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10954 | PDIA5 | protein disulfide isomerase... | NM_006810.4 | 50.1% | 49.7% | (many diffs) |
| 2 | human | 10954 | PDIA5 | protein disulfide isomerase... | NR_028444.2 | 46.5% | 1_140del;927_1688del | |
| 3 | human | 10954 | PDIA5 | protein disulfide isomerase... | XR_427360.1 | 46% | 1_140del;927_1707del | |
| 4 | human | 10954 | PDIA5 | protein disulfide isomerase... | XR_427359.1 | 42.7% | (many diffs) | |
| 5 | mouse | 72599 | Pdia5 | protein disulfide isomerase... | XR_384605.3 | 49.6% | (many diffs) | |
| 6 | mouse | 72599 | Pdia5 | protein disulfide isomerase... | XR_384603.3 | 44.6% | (many diffs) | |
| 7 | mouse | 72599 | Pdia5 | protein disulfide isomerase... | NM_028295.1 | 42.8% | 44.6% | (many diffs) |
| 8 | mouse | 72599 | Pdia5 | protein disulfide isomerase... | XR_384604.3 | 39.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 852
- ORF length:
- 786
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gcgggccggg ccggcgtggc tgctgctggc aatctgggtg gtcctgccat 121 catggctgtc ctctgcaaag gtctcctcgc tcattgagag aatctctgac cccaaggact 181 tgaaaaaact gctcagaacc cggaataatg tactggtgct ttactccaaa tctgaggtgg 241 cagctgaaaa tcatctcagg ttactgtcca cagtggccca ggcggtgaaa ggacaaggga 301 ccatctgctg ggtggactgt ggtgatgcag agagtagaaa attgtgcaag aagatgaaag 361 ttgacctgag cccgaaggac aaaaaggttg aattattcca ttaccaggat ggtgcatttc 421 atactgaata taaccgagct gtgacattta agtccatagt ggcctttttg aaggatccaa 481 aagggccccc actgtgggag gaagatcctG GAGCCAAAGA TGTTGTCCAC CTTGACAGTG 541 AAAAGGACTT CAGACGGCTC CTGAAGAAGG AAGAGAAGCC GCTCCTGATC ATGTTTTATG 601 CCCCCTGGTG CAGCATGTGC AAGAGGATGA TGCCGCATTT CCAGAAGGCT GCGACTCAGC 661 TGCGAGGCCA CGCCGTGCTG GCCGGGATGA ATGTCTACTC CTCTGAATTT GAAAACATCA 721 AGGAGGAGTA CAGCGTGCGC GGCTTCCCCA CCATCTGCTA TTTTGAGAAA GGACGGTTCT 781 TGTTCCAGTA TGACAACTAT GGGTCCACAG CTGAGGACAT TGTGGAGTGG CTGAAGAAGG 841 TGTGGCCACT GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 901 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 961 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAACC ATCTTTTCTA AATGGATCAT 1021 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t