Construct: ORF TRCN0000471427
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004974.1_s317c1
- Derived from:
- ccsbBroadEn_00679
- DNA Barcode:
- CGCACGCATATTTTATCCTCTTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPR39 (2863)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471427
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2863 | GPR39 | G protein-coupled receptor 39 | NM_001508.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1428
- ORF length:
- 1359
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcttcaccc agcctcccgg gcagtgactg ctcccaaatc attgatcaca 121 gtcatgtccc cgagtttgag gtggccacct ggatcaaaat cacccttatt ctggtgtacc 181 tgatcatctt cgtgatgggc cttctgggga acagcgccac cattcgggtc acccaggtgc 241 tgcagaagaa aggatacttg cagaaggagg tgacagacca catggtgagt ttggcttgct 301 cggacatctt ggtgttcctc atcggcatgc ccatggagtt ctacagcatc atctggaatc 361 ccctgaccac gtccagctac accctgtcct gcaagctgca cactttcctc ttcgaggcct 421 gcagctacgc tacgctgctg cacgtgctga cactcagctt tgagcgctac atcgccatct 481 gtcacccctt caggtacaag gctgtgtcgg gaccttgcca ggtgaagctg ctgattggct 541 tcgtctgggt cacctccgcc ctggtggcac tgcccttgct gtttgccatg ggtactgagt 601 accccctggt gaacgtgccc agccaccggg gtctcacttg caaccgctcc agcacccgcc 661 accacgagca gcccgagacc tccaatatgt ccatctgtac caacctctcc agccgctgga 721 ccgtgttcca gtccagcatc ttcggcgcct tcgtggtcta cctcgtggtc ctgctctccg 781 tagccttcat gtgctggaac atgatgcagg tgctcatgaa aagccagaag ggctcgctgg 841 ccgggggcac gcggcctccg cagctgagga agtccgagag cgaagagagc aggaccgcca 901 ggaggcagac catcatcttc ctgaggctga ttgttgtgac attggccgta tgctggatgc 961 ccaaccagat tcggaggatc atggctgcgg ccaaacccaa gcacgactgg acgaggtcct 1021 acttccgggc gtacatgatc ctccTCCCCT TCTCGGAGAC GTTTTTCTAC CTCAGCTCGG 1081 TCATCAACCC GCTCCTGTAC ACGGTGTCCT CGCAGCAGTT TCGGCGGGTG TTCGTGCAGG 1141 TGCTGTGCTG CCGCCTGTCG CTGCAGCACG CCAACCACGA GAAGCGCCTG CGCGTACATG 1201 CGCACTCCAC CACCGACAGC GCCCGCTTTG TGCAGCGCCC GTTGCTCTTC GCGTCCCGGC 1261 GCCAGTCCTC TGCAAGGAGA ACTGAGAAGA TTTTCTTAAG CACTTTTCAG AGCGAGGCCG 1321 AGCCCCAGTC TAAGTCCCAG TCATTGAGTC TCGAGTCACT AGAGCCCAAC TCAGGCGCGA 1381 AACCAGCCAA TTCTGCTGCA GAGAATGGTT TTCAGGAGCA TGAAGTTTTG CCAACTTTCT 1441 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1501 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1561 GTGGAAAGGA CGACGCACGC ATATTTTATC CTCTTTAACG CGTTAAGTCg acaatcaacc 1621 tctggattac aaaatttgtg aaagatt