Construct: ORF TRCN0000471444
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001297.1_s317c1
- Derived from:
- ccsbBroadEn_11492
- DNA Barcode:
- GCACCTCTCACATAGCCTTGTTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDRG1 (10397)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471444
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10397 | NDRG1 | N-myc downstream regulated 1 | XM_011516792.2 | 53.1% | 53.1% | 1_288del |
| 2 | human | 10397 | NDRG1 | N-myc downstream regulated 1 | NM_001258433.1 | 34.8% | 34.8% | 1_612del |
| 3 | human | 10397 | NDRG1 | N-myc downstream regulated 1 | NM_001258432.2 | 33.2% | 33.2% | 1_657del |
| 4 | human | 10397 | NDRG1 | N-myc downstream regulated 1 | NM_001135242.2 | 27.6% | 27.6% | 1_855del |
| 5 | human | 10397 | NDRG1 | N-myc downstream regulated 1 | NM_006096.4 | 27.6% | 27.6% | 1_855del |
| 6 | human | 10397 | NDRG1 | N-myc downstream regulated 1 | XM_011516791.1 | 26.5% | 26.5% | 1_906del |
| 7 | mouse | 17988 | Ndrg1 | N-myc downstream regulated ... | NM_008681.2 | 24.1% | 25.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 91
- ORF end:
- 418
- ORF length:
- 327
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTTCTTC CATTTCAGGT GTCGTGAGGC TAGCATCGAT 61 TGATCAACAA GTTTGTACAA AAAAGTTGGC ATGGCGGACT GTGGCGGCCT CCCGCAGATC 121 TCCCAGCCGG CCAAGCTCGC TGAGGCCTTC AAGTACTTCG TGCAGGGCAT GGGATACATG 181 CCCTCGGCTA GCATGACCCG CCTGATGCGG TCCCGCACAG CCTCTGGTTC CAGCGTCACT 241 TCTCTGGATG GCACCCGCAG CCGCTCCCAC ACCAGCGAGG GCACCCGAAG CCGCTCCCAC 301 ACCAGCGAGG GCACCCGCAG CCGCTCGCAC ACCAGCGAGG GGGCCCACCT GGACATCACC 361 CCCAACTCGG GTGCTGCTGG GAACAGCGCC GGGCCCAAGT CCATGGAGGT CTCCTGCTAC 421 CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT 481 CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT 541 TATATATCTT GTGGAAAGGA CGAGCACCTC TCACATAGCC TTGTTACACG CGTTAAGTCg 601 acaatcaacc tctggattac aaaatttgtg aaagatt