Construct: ORF TRCN0000471460
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010851.1_s317c1
- Derived from:
- ccsbBroadEn_08278
- DNA Barcode:
- CTTCCTACGATTGGGTCACGTAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPL35 (51318)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471460
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51318 | MRPL35 | mitochondrial ribosomal pro... | NM_145644.2 | 99.6% | 98.8% | 55C>T;70G>A |
| 2 | human | 51318 | MRPL35 | mitochondrial ribosomal pro... | NM_001363782.1 | 95.6% | 94.9% | 55C>T;70G>A;511_531del |
| 3 | human | 51318 | MRPL35 | mitochondrial ribosomal pro... | NM_016622.4 | 90% | 89.3% | 55C>T;70G>A;511_564del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 576
- ORF length:
- 510
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcctctgcc tttgctggtg cagtgagagc agcttcagga atcctacggt 121 ccctgaatat tttgacatct tcaacctacc gcaactgtgt caagaatgcc tctcttattt 181 ctgcattgtc cactggacgt tttagtcata ttcagacacc agttgtttcc tccactccca 241 gacttaccac atctgagaga aaccTGACAT GTGGGCATAC CTCAGTGATC CTTAATAGAA 301 TGGCCCCCGT GCTTCCAAGT GTCCTGAAGC TGCCAGTCAG ATCTCTAACA TACTTCAGTG 361 CAAGAAAAGG CAAGAGAAAG ACCGTGAAAG CTGTCATCGA TAGGTTTCTT CGACTTCATT 421 GTGGCCTTTG GGTGAGGAGA AAGGCTGGCT ATAAGAAAAA ATTATGGAAA AAGACACCTG 481 CAAGGAAGAA GCGATTGAGG GAATTTGTAT TCTGCAATAA AACCCAGAGT AAACTCTTAG 541 ATAAAATGAC GACGTCCTTC TGGAAGAGGC GAAACTGCCC AACTTTCTTG TACAAAGTGG 601 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 661 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 721 ACTTCCTACG ATTGGGTCAC GTAGTACGCG TTAAGTCgac aatcaacctc tggattacaa 781 aatttgtgaa agatt