Construct: ORF TRCN0000471466
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006836.1_s317c1
- Derived from:
- ccsbBroadEn_11859
- DNA Barcode:
- TATTCGTGACTAAGTATAAGCCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLHL20 (27252)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471466
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27252 | KLHL20 | kelch like family member 20 | XM_011509420.3 | 38.9% | 35.4% | (many diffs) |
| 2 | human | 27252 | KLHL20 | kelch like family member 20 | XM_017001054.2 | 38.9% | 35.4% | (many diffs) |
| 3 | human | 27252 | KLHL20 | kelch like family member 20 | NM_014458.4 | 35.9% | 32.7% | (many diffs) |
| 4 | human | 27252 | KLHL20 | kelch like family member 20 | XM_005245093.4 | 35.9% | 32.7% | (many diffs) |
| 5 | human | 27252 | KLHL20 | kelch like family member 20 | XM_017001053.1 | 34.3% | 31.3% | (many diffs) |
| 6 | human | 27252 | KLHL20 | kelch like family member 20 | XM_017001052.1 | 31.9% | 29.2% | (many diffs) |
| 7 | human | 27252 | KLHL20 | kelch like family member 20 | XR_001737119.1 | 23.8% | (many diffs) | |
| 8 | human | 27252 | KLHL20 | kelch like family member 20 | XR_921767.3 | 20.2% | (many diffs) | |
| 9 | human | 27252 | KLHL20 | kelch like family member 20 | XR_001737121.2 | 19.4% | (many diffs) | |
| 10 | human | 27252 | KLHL20 | kelch like family member 20 | XR_921766.3 | 18.9% | (many diffs) | |
| 11 | human | 27252 | KLHL20 | kelch like family member 20 | XR_001737120.1 | 18.6% | (many diffs) | |
| 12 | mouse | 226541 | Klhl20 | kelch-like 20 | NM_001039482.1 | 33.1% | 31.9% | (many diffs) |
| 13 | mouse | 226541 | Klhl20 | kelch-like 20 | XR_373601.3 | 18.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 756
- ORF length:
- 690
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga aggaaagcca atgcgcaggt gtaccaacat tcgaccagga gagactggaa 121 tggatgtaac aagccgctgc acccttggag accccaacaa actgccagaa ggggttcccc 181 aacctgcccg catgccctat atctcagaca agcaccctcg acaaaccttg gaagtgatta 241 accTTCTGAG AAAGCACCGG GAGCTATGTG ATGTGGTGCT AGTTGTGGGC GCCAAGAAGA 301 TATATGCCCA TCGAGTCATT TTGTCAGCCT GTAGTCCCTA CTTCCGAGCT ATGTTTACAG 361 GAGAATTGGC AGAGAGCCGT CAGACAGAAG TAGTGATCCG AGACATTGAC GAGAGGGCTA 421 TGGAATTACT GATTGACTTT GCGTATACCT CCCAGATAAC AGTAGAAGAG GGCAATGTTC 481 AGACTCTTCT GCCAGCTGCT TGCCTCCTCC AGCTGGCAGA AATACAGGAA GCCTGCTGTG 541 AATTCTTAAA GAGACAATTA GATCCTTCTA ACTGCCTGGG CATTCGGGCT TTTGCTGACA 601 CACATTCATG TCGTGAGTTG CTAAGGATAG CAGACAAGTT CACCCAACAT AACTTTCAAG 661 AGGTGAGTTG CACTTGGTGT TCCAATGCAC CCATTTGTTG GAGAGCAATA TGGGTTCACA 721 TTCTGCTAAA TAACACTTGC CTGGGTATTT CCTGCTGCCC AACTTTCTTG TACAAAGTGG 781 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 841 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 901 ATATTCGTGA CTAAGTATAA GCCCCACGCG TTAAGTCgac aatcaacctc tggattacaa 961 aatttgtgaa agatt