Construct: ORF TRCN0000471471
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018207.1_s317c1
- Derived from:
- ccsbBroadEn_10273
- DNA Barcode:
- CGCTACCAGCGGATCATGTATGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GLIS3-AS1 (84850)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471471
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84850 | GLIS3-AS1 | GLIS3 antisense RNA 1 | NR_026663.1 | 23.8% | 1_166del;341_730del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 222
- ORF end:
- 396
- ORF length:
- 174
- Sequence:
-
1 GGAGGGGTTT TATGCGATGG AGTTTCCCCA CACTGAGTGG GTGGAGACTG AAGTTAGGCC 61 AGCTTGGCAC TTGATGTAAT TCTCCTTGGA ATTTGCCCTT TTTGAGTTTG GATCTTGGTT 121 CATTCTCAAG CCTCAGACAG TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAGG 181 CTAGCATCGA TTGATCAACA AGTTTGTACA AAAAAGTTGG CATGGAGCTC TGTGCTGGAC 241 CGCAACTAAG AGGACAAAAC GGAAGAGACA TCGATAAGGA GAGCCGGTCC TTGGAATATT 301 TTCCTGGGAA GGCATCATGC TCTATCAGTG CAACTCAGCC CACAAAAATT TATCAAGCAG 361 CAACTACGGG TCCTCTGAAC GGGCCACACC ACGGTTGCCC AACTTTCTTG TACAAAGTGG 421 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 481 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 541 ACGCTACCAG CGGATCATGT ATGCAACGCG TTAAGTCgac aatcaacctc tggattacaa 601 aatttgtgaa agatt