Construct: ORF TRCN0000471472
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013044.1_s317c1
- Derived from:
- ccsbBroadEn_10317
- DNA Barcode:
- AATCTAACATGAGTGGTCACGTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC158435 (158435)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471472
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 158435 | LOC158435 | uncharacterized LOC158435 | NR_033838.1 | 6.9% | 1_1671del;1843_2464del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 221
- ORF end:
- 392
- ORF length:
- 171
- Sequence:
-
1 GAGGGGTTTT ATGCGATGGA GTTTCCCCAC ACTGAGTGGG TGGAGACTGA AGTTAGGCCA 61 GCTTGGCACT TGATGTAATT CTCCTTGGAA TTTGCCCTTT TTGAGTTTGG ATCTTGGTTC 121 ATTCTCAAGC CTCAGACAGT GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAGGC 181 TAGCATCGAT TGATCAACAA GTTTGTACAA AAAAGTTGGC ATGGATTTGC AGCAGGCTGG 241 TGGAGGCAGA GCAGAGAAGG GCGGGAGAGG GCCAGCGAAG GCCTGGGAAG GTGGACCCAC 301 AAACTCGTCT CCTAATGAAG CTAACCAGCA ATGCGAGGGC ACACAGGGAG ACCCCCTAGA 361 GGCTGCCCCA GGCCAGGAGT CACCTGTGAA CTGCCCAACC TTCTTGTACA AAGTGGTTGA 421 TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG 481 TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAAT 541 CTAACATGAG TGGTCACGTT GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt 601 tgtgaaagat t